Gli3 Antibody

• Catalog number
  R30859
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  Gli3
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB
• Recommended dilutions
  Western blot: 0.5-1ug/ml (1)
• Notes
  The stated application concentrations are suggested starting amounts. Titration of the Gli3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. There are two isoforms of Gli-kruppel family member 3: the 170-190KD full length form and a truncated ~80KD form. This antibody will detect both, depending on the testing material.
• Intented use
  This Gli3 antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  P10071
• Purity
  Antigen affinity
• Description
  Gli-kruppel family member 3 is a member of the zinc finger family related to Kruppel, a protein family that is known to regulate development in Drosophila. The gene is expressed as an 8.5-kb mRNA in tissues such as testis, myometrium, placenta, and lung, and the protein product (relative molecular mass, 190,000D) shows sequence-specific DNA binding. Gli3 is homologous to the Drosophila cubitus interruptus (ci) gene product(Ci), which regulates the Patched (pct), Gooseberry (gsb), and Decapentaplegic (dpp) genes. PKA-dependent processing of vertebrate Gli3 in developing limb generates a potent repressor in a manner antagonized by apparent long-range signaling from posteriorly localized Sonic hedgehog protein. The high relative abundance and potency of Gli3 repressor suggested specialization of the protein and its products for negative hedgehog pathway regulation.
• Immunogen
  An amino acid sequence from the N-terminus of human Gli-kruppel family member 3 (SNEDESPGQTYHRERRNA) was used as the immunogen for this Gli3 antibody.
• Storage
  After reconstitution, the Gli3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  Gli3  
• Gene symbol
  GLI3
• Short name
  Anti-Gli3
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to Gli3
• Alternative technique
  antibodies
• Alternative to gene target
  GLI family zinc finger 3, ACLS and GCPS and GLI3-190 and GLI3FL and PAP-A and PAPA and PAPA1 and PAPB and PHS and PPDIV, GLI3 and IDBG-13536 and ENSG00000106571 and 2737, metal ion binding, nuclei, Gli3 and IDBG-131686 and ENSMUSG00000021318 and 14634, GLI3 and IDBG-634502 and ENSBTAG00000010671 and 785371

Gene info

• Identity
  HGNC:4319
• Gene
  GLI3
• Long gene name
  GLI family zinc finger 3
• Synonyms gene
  • GCPS
  • PHS
• Synonyms gene name
  • Greig cephalopolysyndactyly syndrome
  • GLI-Kruppel family member GLI3
  • glioma-associated oncogene family zinc finger 3
• Synonyms
  • PAP-A
  • PAPA
  • PAPA1
  • PAPB
  • ACLS
  • PPDIV
• Synonyms name
  • zinc finger protein GLI3
  • oncogene GLI3
  • DNA-binding protein
• Locus
  7p14.1
• Discovery year
  1989-05-29
• Entrez gene record
  2737
• Pubmed identfication
  • 2118997
• RefSeq identity
  • NM_000168
• Classification
  • Zinc fingers C2H2-type
• VEGA ID
  OTTHUMG00000023630

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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