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Also known as
Nerve Growth Factor Receptor, p75NTR- FITC
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Other names
nerve growth factor receptor; N/A; nerve growth factor receptor; NRH; nerve growth factor receptor (TNFR superfamily, member 16); nerve growth factor receptor
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
ngfr; N/A; nrh1; CD271; NRH1b; p75ntr; p75(NTR); tnfrsf16; MGC131034; Gp80-LNGFR; N/A
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Gene name synonims
ngfr; N/A; nrh1; CD271; NRH1b; p75ntr; p75(NTR); tnfrsf16; MGC131034; Gp80-LNGFR; N/A
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Other gene names
ngfr; N/A; nrh1; CD271; NRH1b; p75ntr; p75(NTR); tnfrsf16; MGC131034; Gp80-LNGFR; N/A
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Clonality
Monoclonal
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Immunoglobulin isotype
IgG2A
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Clone
9G397
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Host organism
Mouse (Mus musculus)
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Source organism
Rat (Rattus norvegicus)
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Species reactivity
Rat (Rattus norvegicus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
Recognizes the extracellular domain of the neurotrophin receptor p75NTR in rat. May be used for immunocytochemical localisation of rat cells expressing p75NTR, ELISA and western blot. This antibody has also been used for the construction of the MC192-saporin immunotoxin for specific elimination of neuronal populations in basal forebrain cholinergic neurons to generate an animal model for Alzheimer's disease. Does not react with mouse; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
Affinity Purified by affinity chromatography.
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Form Appearance
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with fluorescein isothiocyanate (FITC).
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Concentration
N/A
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Storage and shipping
For long-term storage and to avoid repeated freezing and thawing, aliquot and For optimal long term storage, the antibody should be kept at -20 degrees Celsius. Aliquots are stable for at least 12 months the antibody should be stored at -20 degrees Celsius.. Prior to use, briefly centrifuge the original vial after thawing and before lid. If further dilution(s) are needed, they can be done with the appropriate assay buffer. FITC conjugates are sensitive to light.
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Tested applications
Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
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Test
A high affinity purification column was use to purify Nerve Growth Factor Receptor, p75NTR - FITC ( - NGF receptor, Gp80-LNGFR, p75 ICD, neurotrophin receptor p75NTR) by MBS Monoclonals by chromatographic size exclusion.
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Description
Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells. The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
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Properties
This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Gene target
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Gene symbol
NGF, NGF-AS1
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Short name
Nerve Growth Factor Receptor, p75NTR - FITC ( - NGF receptor, Gp80-LNGFR, p75 ICD, neurotrophin receptor p75NTR)
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Technique
affinity, FITC, Fluorescein
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Label
FITC
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Alternative name
Anti-Nerve Growth Factor Receptor, p75NTR - FITC (Low-affinity NGF receptor, Gp80-LNGFR, p75 ICD, Low affinity neurotrophin receptor p75NTR)
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Alternative technique
fluorescine
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Alternative to gene target
nerve growth factor (beta polypeptide), Beta-NGF and HSAN5 and NGFB, NGF and IDBG-101347 and ENSG00000134259 and 4803, growth factor activity, Extracellular, Ngf and IDBG-180362 and ENSMUSG00000027859 and 18049, NGF and IDBG-648895 and ENSBTAG00000007446 and 281350
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Tissue
nerve
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