Chicken anti Goat IgG H + L FITC

• Catalog number
  43R-1035
• Price
  Please ask
• Size
  1 mg

• Category
  Secondary Antibody
• Antibody Subtype
  Conjugated Secondary Antibodies
• Area of research
  Immunology
• Type of Immunogen
  Purified Goat IgG (H&L)
• Raised in
  Chicken
• Specificity
  This antibody reacts with heavy chains on Goat IgG and light chains on all Goat immunoglobulins.
• Cross Reactivity
  No reactivity is observed to non-immunoglobulin Goat serum proteins.
• Conjugate Tag
  FITC
• Grade Purity
  Grater than 95% pure
• Method of Purification
  Affinity purified using solid phase Goat IgG (H&L)
• Source
  Ig fraction was prepared using eggs obtained from healthy hens of US origin.
• Concentration
  1.0 mg/ml (lot specific)
• Form Buffer
  Clear, fluorescent yellow liquid, containing 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, 1% (w/v) BSA and 0.05% (w/v) NaN3. Protease/IgG free, pH 7.2.
• Storage
  Store at 2-8 deg C.
• Shipping conditions
  Blue Ice
• Tested for
  FC; IF; IHC
• Usage Recommendations
  The optimal working dilution should be determined by the end user. Suggested starting dilution(s): 1:20 – 1:2000 for most applications
• URL
• More info
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
• Properties
  Chickens like all bird species have IgY antibodies that are very stable and found in the yolk. Gallus Gallus domestica is the Latin name of the Chicken. This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• Latin name
  Capra aegagrus hircus
• About
  Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.

• Gene target
  -
• Gene symbol
  CALD1, FUT1, L3MBTL2
• Short name
  anti Goat IgG H + L FITC
• Technique
  anti, FITC, Goat, IgG, antibody to, Fluorescein, goats, IgGs
• Host
  Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
• Isotype
  IgG, IgG
• Label
  FITC
• Species
  chicken, Chickens, Goats
• Alternative name
  Chicken anti Goat IgG (H + L) secondary antibody (FITC)
• Alternative technique
  antibodies, caprine, fluorescine, immunoglobulins

Gene info

• Identity
  HGNC:1441
• Gene
  CALD1
• Long gene name
  caldesmon 1
• Synonyms
  • CDM
  • H-CAD
  • L-CAD
• GenBank acession
  • M64110
• Locus
  7q33
• Discovery year
  1992-07-20
• Entrez gene record
  800
• Pubmed identfication
  • 1885618
• RefSeq identity
  • NM_033138
• VEGA ID
  OTTHUMG00000155407

Gene info

• Identity
  HGNC:4012
• Gene
  FUT1
• Long gene name
  fucosyltransferase 1 (H blood group)
• Synonyms gene
  • H
  • HSC
• Synonyms gene name
  • fucosyltransferase 1 (galactoside 2-alpha-L-fucosyltransferase, Bombay phenotype included)
  • fucosyltransferase 1 (galactoside 2-alpha-L-fucosyltransferase)
• Synonyms name
  • galactoside 2-alpha-L-fucosyltransferase
• Locus
  19q13.33
• Discovery year
  1989-06-06
• Entrez gene record
  2523
• RefSeq identity
  • NM_000148
• Classification
  • Fucosyltransferases
  • Blood group antigens
• VEGA ID
  OTTHUMG00000183325
• Locus Specific Databases
  • Blood Group Antigen Mutation Database
  • LRG_810

Gene info

• Identity
  HGNC:18594
• Gene
  L3MBTL2
• Long gene name
  L3MBTL histone methyl-lysine binding protein 2
• Synonyms gene name
  • l(3)mbt-like 2 (Drosophila)
  • L3MBTL2, polycomb repressive complex 1 subunit
• Synonyms
  • H-l(3)mbt-l
  • DKFZP761I141
  • dJ756G23.3
• GenBank acession
  • AJ305226
• Locus
  22q13.2
• Discovery year
  2002-04-29
• Entrez gene record
  83746
• Pubmed identfication
  • 11682070
  • 21596310
• RefSeq identity
  • NM_031488
• Classification
  • MBT domain containing
• VEGA ID
  OTTHUMG00000150942

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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