Chicken anti Rabbit IgG FITC

• Catalog number
  43R-1601
• Price
  Please ask
• Size
  1 ml

• Category
  Secondary Antibody
• Antibody Subtype
  Conjugated Secondary Antibodies
• Area of research
  Immunology
• Type of Immunogen
  Chicken anti-Rabbit IgG (FITC) was raised in Chicken using purified, non-denatured rabbit IgG (containing both heavy and light chains) ..
• Raised in
  Chicken
• Specificity
  Rabbit IgG
• Conjugate Tag
  FITC
• Method of Purification
  Chicken anti-Rabbit IgG (FITC) was purified by affinity chromatography.
• Concentration
  1 mg/ml
• Form Buffer
  Provided in liquid form in 10 mM PBS buffer, pH 7.2, with 0.02% NaN3 added as an antimicrobial agent and 0.2 % BSA.
• Storage
  Store at 4 deg C for short term storage. For long term storage add glycerol, aliquot and store at -20 deg C. Avoid repeated freeze/thaw cycles. Protect from light.
• Shipping conditions
  Blue Ice
• Tested for
  Please refer to the datasheet
• URL
• More info
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
• Properties
  Chickens like all bird species have IgY antibodies that are very stable and found in the yolk. Gallus Gallus domestica is the Latin name of the Chicken. This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• About
  Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rabbits are used for polyclonal antibody production by fitzgerald. Rabbit antibodies are very stable and can be stored for several days at room temperature. fitzgerald adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
• Latin name
  Oryctolagus cuniculus

• Short name
  anti Rabbit IgG FITC
• Technique
  Rabbit, anti, FITC, IgG, antibody to, Fluorescein, IgGs
• Host
  Rabbit, Rabbits
• Isotype
  IgG, IgG
• Label
  FITC
• Species
  chicken, Chickens
• Alternative name
  Chicken anti Rabbit IgG secondary antibody (FITC)
• Alternative technique
  rabbit-anti, antibodies, fluorescine, immunoglobulins

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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