Chicken anti Human IgG FITC
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Catalog number
43R-1599
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Price
Please ask
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Size
1 ml
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Category
Secondary Antibody
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Antibody Subtype
Conjugated Secondary Antibodies
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Area of research
Immunology
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Type of Immunogen
Chicken anti-Human IgG (FITC) was raised in Chicken using purified, non-denatured human IgG (containing both heavy and light chains) ..
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Raised in
Chicken
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Specificity
Human IgG
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Conjugate Tag
FITC
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Method of Purification
Chicken anti-Human IgG (FITC) was purified by affinity chromatography.
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Concentration
1 mg/ml
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Form Buffer
Provided in liquid form in 10 mM PBS buffer, pH 7.2, with 0.02% NaN3 added as an antimicrobial agent.
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Storage
Store at 4 deg C for short term storage. For long term storage add glycerol, aliquot and store at -20 deg C. Avoid repeated freeze/thaw cycles. Protect from light.
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Shipping conditions
Blue Ice
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Tested for
Please refer to the datasheet
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
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Properties
Chickens like all bird species have IgY antibodies that are very stable and found in the yolk. Gallus Gallus domestica is the Latin name of the Chicken. This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Conjugation
Anti-FITC Antibody
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
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Short name
anti IgG FITC
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Technique
anti human, anti, FITC, IgG, antibody to, anti human antibodies, Fluorescein, IgGs
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Isotype
IgG, IgG
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Label
FITC
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Species
Human, Chickens, Humans
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Alternative name
Chicken anti Human IgG secondary antibody (FITC)
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Alternative technique
antibodies, fluorescine, immunoglobulins
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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