IgG, Murine Isotype Control (FITC)
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Catalog number
MBS655601
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Price
Please ask
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Size
0,5 mg
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Other size
please contact us to order other different size
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Description
Isotype or positive controls by peptides, antibodies and deactivated samples.
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Properties
This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes. Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.
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Conjugation
Anti-FITC Antibody
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
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Group
isotypes
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Gene target
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Gene symbol
ARAFP2, BRAFP1
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Short name
IgG, Murine Isotype Control (FITC)
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Technique
Control, Isotype, FITC, IgG, controls, Fluorescein, IgGs, Isotypes and controls
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Isotype
IgG, IgG
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Label
FITC
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Alternative name
Immunoglobulin G, Murine isoform reference (fluorecein)
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Alternative technique
control, controls, fluorescine, immunoglobulins
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Tissue
control
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Gene info
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Identity
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Gene
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Long gene name
ARAF pseudogene 2
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Synonyms gene
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Synonyms gene name
- v-raf murine sarcoma 3611 viral oncogene homolog pseudogene
- v-raf murine sarcoma 3611 viral oncogene homolog 2, pseudogene
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Synonyms
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Locus
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Discovery year
1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
BRAF pseudogene 1
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Synonyms gene
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Synonyms gene name
- v-raf murine sarcoma viral oncogene homolog B pseudogene 2
- v-raf murine sarcoma viral oncogene homolog B pseudogene 1
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Synonyms
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Locus
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Discovery year
2003-07-21
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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