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Category
Primary Antibodies
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Long description
FAK is a widely expressed non-receptor protein tyrosine kinase discovered as substrate for Src and as a key element of integrin signaling. Regulation of FAK include phosphorylation at multiple tyrosine and serine residues. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Phosphorylation of tyrosine generally is associated with positive regulation and growth promotion; however, dephosphorylation at these sites occurs as cells begin to enter the M-phase of the cell cycle. In contrast, serine phosphorylation either remains high or is increased as cells enter mitosis. FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. FAK binds multiple signaling proteins including p130Cas, Graf, Grb2, Src family SH2 domains, and the p85 subunit of PI3-kinase.
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Antibody come from
Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with swine recombinant IL-2 protein and NSO mouse myeloma cells.
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Other description
Provided as solution in phosphate buffered saline, pH 7.3, with 0.5% BSA and 0.05% sodium azide. Purified from ascites by affinity chromatography.
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Clone
34Q36
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Antigen antibody binding interaction
Mouse anti Focal Adhesion Kinase (FAK) Antibody
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Antibody is raised in
Mouse
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Antibody s reacts with
Human, Mouse, Rat
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Western Blot
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Antibody s suited for
Antibody can be used for ELISA as a detector antibody (0.1-1 µg/ml). Just prior to use in ELISA as a detection antibody, we recommend that the antibody be diluted to a concentration of 0.1-1.0 µg/mL in an appropriate buffer, such as PBS containing 0.1-1.0% BSA. Pipette 50 µL of the diluted antibody into each well of a microtiter plate. The optimal concentration should be determined for each specific application. A general ELISA procedure is available upon request.
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Storage
-20ºC
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Relevant references
Abrams, J.S., M.G. Roncarolo, H. Yssel, U. Andersson, G.J. Gleich, and J.E. Silver (1992) Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunological Review 127:5-24.
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
cell adhesion molecules play a role in cell growth and activation and are often identified by WB or ELISA as in the Mouse anti Focal Adhesion Kinase (FAK). This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
