Cystathionine β Synthase Activity Assay Kit (Fluorometric)

• Catalog number
  K998-100
• Price
  Please ask
• Size
  100 assays

• Description
  First assay in the market that measures CβS activity in biological samples
• Summary
  • Detection method - Fluorescence (Ex/Em 368/460 nm) • Species reactivity - Eukaryotes • Applications - Rapid assessment of native/recombinant CβS and CβS activity in mammalian samples
• Detection Method
  Fluorescence (Ex/Em 368/460 nm)
• Species Reactivity
  Eukaryotes
• Applications
  Rapid assessment of native/recombinant CβS and CβS activity in mammalian samples
• Sample Type
  Mammalian tissues and cell cultures · Purified or recombinant CβS
• Features Benefits
  · Simple, convenient, highly sensitive ·  Fluorescent assay enables easy determination of CβS activity in a variety of biological samples · Kit includes a stable CβS positive control
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Cystathionine β Synthase (EC 4.2.1.22, CβS) is a PLP-dependent enzyme that catalyzes the formation of H2S and cystathionine when using cysteine and homocysteine as substrates. Known for its role in human sulfur metabolism, mutations in the gene encoding CβS can result in high concentrations of homocysteine in plasma. Elevated circulating concentrations of homocysteine result in genetic disorders including Homocysteinuria and Down syndrome. Specifically, Homocysteinuria is an autosomal recessive disease with clinical manifestations including mental retardation, thromboembolism and connective tissue defects. Hydrogen sulfide, CβS product, is an important gaseous mediator, like nitric oxide, that has significant effects on the immunological, neurological, cardiovascular and pulmonary systems of mammals. BioVision’s Cystathione β Synthase Assay Kit utilizes cysteine and homocysteine as substrates to produce H2S. Hydrogen sulfide reacts with the azido-functional group of the fluorescent probe yielding a fluorescent amino group (Ex/Em = 368/460 nm). The assay is highly sensitive, has a simple easy-to-follow protocol, and can detect as low as 1.45 mU of CβS activity.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.

• Gene target
  Cystathionine   Synthase   Activity   Kit   Fluorometric  
• Short name
  Cystathionine β Synthase Activity Assay Kit (Fluorometric)
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  Cystathionine β Synthase Activity test reagent (fluorescence)
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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