Rabbit secondary antibody to sheep IgG (H+L)-Biotin
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Catalog number
MBS355679
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Price
Please ask
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Size
0.1 mL
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Products_type
Secondary Antibody
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Products_short_name
[Rabbit anti-sheep IgG]
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Products_name_syn
[Rabbit anti-sheep IgG (H+L)-Biotin conjugated]
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Reactivity
Sheep
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Specificity
It can specifically recognize the heavy chains on sheep IgG and the light chains on all sheep immunoglobulins. And Immunoelectrophoresis shows bands only in the gamma region against normal sheep serum.
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Purity
This product was purified from antiserum by immunoaffinity chromatography.
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Form
Liquid
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Storage_stability
Store at 4 degree C for frequent use, at -20 degree C for long term. And store the diluted solution at 4 degree C. Avoid repeated freeze and thaw cycles.
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Tested_application
Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), ELISA (EIA)
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Properties
If you buy Antibodies supplied by MyBioSource they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Conjugation
Biotinylated
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rabbits are used for polyclonal antibody production by MyBioSource. Rabbit antibodies are very stable and can be stored for several days at room temperature. MyBioSource adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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French translation
anticorps
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Gene target
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Short name
Rabbit secondary antibody IgG (H+L)-
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Technique
Antibody, Rabbit, IgG, antibodies against human proteins, antibodies for, IgGs
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Host
Rabbit, Rabbits
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Isotype
IgG, IgG
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Label
Biotin
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Species
Sheep, The sheep (Ovis aries) ELISAs are detecting sheep proteins and antigens. Some epitopes will also recognize goat proteins. Sheep and goats are also used to produce polyclonals. An adult female sheep is referred to as a ewe a male as a ram, a castrated male as a wether, and a younger sheep as a lamb. Sheeps
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Alternative name
production species: rabbit secondary detector Antibody (antibody to-) to ovine Immunoglobulin G (heavy + light chain)-biotinilated
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Alternative technique
antibodies, rabbit-anti, immunoglobulins
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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