anti-TGFb1 Antibody [M66-4B]
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Catalog number
XP-5729-M
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Price
Please ask
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Size
0.5 mg
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Gene names
APO2L, TRAIL, Tumor necrosis factor ligand superfamily member 10, Apo-2 ligand, Apo-2L, TL2, CD253
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Antibodie s label
unlabelled serum
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Purity
This IgG1k antibody was purified on a Protein G affinity column.
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Ab raised in
Mouse
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Type
Monoclonal
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Monoclonal
M66-4B
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Antigen
The monoclonal antibody was produced from a hybridoma using purified recombinant, insect cell derived, hTGFβ1.
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Immuno application
ELISA, Western Blot
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Antibody advice
ELISA:In a sandwich ELISA (assuming 100 μL/well), a concentration of 4.0-8.0 ug/mL of this antibody will detect at least 100 pg/mL of recombinant human TGFβ1 when used in conjunction with compatible secondary reagents. Western Blot:To detect hTGFβ1 by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTGFβ1 is 2.0-4.0 ng/lane, under non-reducing conditions. In a dot blot, a 1:500 dilution of this antibody exhibited complete cross reactivity with human TGFβ2 and TGFβ3 isotypes. Reduced cross reactivity was shown at a 1:1000 dilution.
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Keep at
TGFb1 lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20 grades C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4 grades C when reconstituted.
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Works with
Human
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Omim nr
1730015
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Ncbi nr
P50591
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Vial with antibody
Lyophilized
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NCBI GENE nr
8743
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GENE symbol
TNFSF10
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Antigene name
tumor necrosis factor (ligand) superfamily, member 10
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Source
Homo sapiens
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Protein nr
P50591
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
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Gene target
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Gene symbol
IGHD4OR15-4B
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Short name
anti-TGFb1 Antibody [M66-4B]
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Technique
Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
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Alternative name
Antibody toTGFb1 (antibody to-) [M66-4B]
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Alternative technique
antibodies
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Alternative to gene target
transforming growth factor, beta 1, CED and DPD1 and LAP and TGFB and TGFbeta, TGFB1 and IDBG-52846 and ENSG00000105329 and 7040, protein N-terminus binding, nuclei, Tgfb1 and IDBG-158292 and ENSMUSG00000002603 and 21803, TGFB1 and IDBG-638417 and ENSBTAG00000020457 and 282089
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Gene info
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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