DNAse I Activity Assay Kit (Fluorometric)

• Catalog number
  K429-100
• Price
  Please ask
• Size
  100 assays

• Description
  High-throughput compatible, quick and easy screening for DNAse activity in biological samples; Includes DNAse positive control
• Summary
  • Detection method - Fluorescence (Ex/Em 651/681 nm) • Species reactivity - Eukaryotes • Applications - Measurement of DNAse activity in various biological samples.
• Detection Method
  Fluorescence (Ex/Em 651/681 nm)
• Species Reactivity
  Eukaryotes
• Applications
  • Measurement of DNAse I activity of purified proteins • Quantitative analysis of DNAse I mutants and inhibitors • Comparative examination of DNAse I activity in biological samples
• Sample Type
  • Measurement of DNAse I activity of purified proteins
• Features Benefits
  Quantitative analysis of DNAse I mutants and inhibitors
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Deoxyribonuclease I (DNAse I) is an endonuclease that cleaves DNA phosphodiester bonds yielding 5′-phosphorylated and 3′-hydroxylated oligonucleotides. DNAse I targets single-stranded DNA, double-stranded DNA, and chromatin in a non-specific manner. As an important player in cellular waste management, DNAse I is normally secreted extracellularly to clear the system from circulating cell-free DNA, foreign DNA from food digestion or potential pathogens, and endogenous chromosomal DNA from apoptotic and necrotic cells. Abnormal DNAse I activity occurs in association with a variety of cancers and auto-immune illnesses that exhibit elevated levels of cell-free DNA. Furthermore, DNAse I has been therapeutically used in cystic fibrosis patients to degrade DNA and reduce sputum viscosity. BioVision’s DNAse I Activity Fluorometric Assay Kit allows for quantitative evaluation of DNAse I activity of purified enzymes and their inhibitors as well as comparative examination of DNAse I activity in biological samples. Enzyme activity is detected upon cleavage of a DNA Probe, which yields a fluorescent DNA product measured at Ex/Em = 651/681 nm. The limit of quantification (L.O.Q) is 178 fmoles of DNA probe cleaved per minute per ml
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.

• Gene target
  DNAse   Activity   Kit   Fluorometric  
• Short name
  DNAse I Activity Assay Kit (Fluorometric)
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  DNAse I Activity test reagent (fluorescence)
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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