anti-IL-8 Antibody

  • Catalog number
    XP-5199Bt
  • Price
    Please ask
  • Size
    0.05 mg
  • Gene info
    IL-8 is a member of the CXC chemokine family. This family of small basic heparan-binding proteins are proinflammatory and primarily mediate the activation and migration of neutrophils into tissue from peripheral blood. This chemokine is one of the major mediators of the inflammatory response and is secreted by several cell types in response to an inflammatory stimulus. It functions as a chemoattractant, and is also a potent angiogenic factor. IL-8 attracts neutrophils, basophils, and T-cells, but not monocytes.
  • Gene names
    NAF, GCP1, LECT, LUCT, NAP1, CXCL8, GCP-1, LYNAP, MDNCF, MONAP, NAP-1, Interleukin-8, C-X-C motif chemokine 8, IL-8
  • Antibodie s label
    Biotin
  • Purity
    Anti-hIL-8 specific antibody was purified by affinity chromatography and then biotinylated.
  • Ab raised in
    Rabbit
  • Type
    Polyclonal
  • Antigen
    Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-8 (human Interleukin-8).
  • Immuno application
    ELISA, Western Blot
  • Antibody advice
    ELISA:To detect hIL-8 by direct ELISA (using 100 μL/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 ug/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hIL-8. Sandwich:To detect hIL-8 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 ug/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human IL-8 (XP-5199) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-8. Western Blot:To detect hIL-8 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-8 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
  • Keep at
    IL-8 antibody is stable for at least 2 years from date of receipt at -20 grades C. The reconstituted antibody is stable for at least two weeks at 2-8 grades C. Frozen aliquots are stable for at least 6 months when stored at -20 grades C. Avoid repeated freeze-thaw cycles.
  • Works with
    Human
  • Omim nr
    124359
  • Ncbi nr
    P10145
  • Vial with antibody
    Lyophilized
  • NCBI GENE nr
    3576
  • GENE symbol
    IL8
  • Antigene name
    interleukin 8
  • Source
    Homo sapiens
  • Protein nr
    P10145
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Properties
    If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
  • French translation
    anticorps
  • Gene target
    IL-8  
  • Gene symbol
    SNORD114-8, IL36G, IL25, RNU6-8, IL22, SNORD113-8, IL18, IL37, SNORD116-8, IGKV2OR2-8
  • Short name
    anti-IL-8 Antibody
  • Technique
    Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
  • Alternative name
    Antibody toIL-8 (antibody to-)
  • Alternative technique
    antibodies
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    immunoglobulin kappa variable 2/OR2-8 (pseudogene)
  • Synonyms gene name
    • immunoglobulin kappa variable 2/OR2-8
    • immunoglobulin kappa variable 2/OR2-8 pseudogene
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2000-04-18
  • Entrez gene record
  • RefSeq identity
  • Classification
    • Immunoglobulin kappa (IGK) orphons
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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