Factor IXa Activity Assay Kit (Fluorometric)
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Catalog number
K364-100
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Price
Please ask
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Size
100 assays
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Description
Simple, Highly Sensitive Assay
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Summary
• Detection method- Fluorescence (Ex/Em = 360/450 nm • Applications: - Determine activity of purified Factor IXa - Detect the activity of Factor IXa in plasma and serum samples
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Detection Method
Fluorescence (Ex/Em = 360/450 nm
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Applications
• Determine activity of purified Factor IXa • Detect the activity of Factor IXa in plasma and serum samples
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Sample Type
Purified Factor Ixa, Plasma, Serum
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Features Benefits
• Rapid and convenient • The assay can detect Factor IXa activity as low as 10 pg.
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Storage Conditions
-20°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
The coagulation Factor IX (or Christmas factor) (EC 3.4.21.22) is a vitamin K-dependent serine protease. Factor IX is produced as an inactive precursor and is activated via cleavage by either factor XIa (contact pathway) or factor VIIa (tissue factor pathway). In the presence of calcium ions and negatively charged membrane phospholipids, activated factor IX (FIXa) then binds to the activated Factor VIII (FVIIIa) and proteolytically activates factor X (FX) to factor Xa (FXa). BioVision’s Factor IXa Activity Assay kit is based on the ability of FIXa to generate FXa. The generated FXa proteolytically cleaves a synthetic substrate and releases a fluorophore, AMC, which can be easily quantified by fluorescence microplate reader. The assay is simple, rapid and can detect activity as low as 10 pg of FIXa in a variety of samples.
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Test
Biovision supplies other types of Assays as 1.
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Additional description
Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
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Gene target
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Short name
Factor IXa Activity Assay Kit (Fluorometric)
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Technique
Assay, assays
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Host
Assay
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Alternative name
Factor IXa Activity test reagent (fluorescence)
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Alternative technique
arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Product images
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