Goat IgG, conjugated with FITC, Isotype control

  • Catalog number
    GIgG-FITC
  • Price
    Please ask
  • Size
    1ml
  • Host Source
    Goat
  • Clone Name
    NA
  • Product Category
    Veterinary
  • Species Reactivity
    Caprine
  • Application
    ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence
  • Immunogen
    NA
  • Purification Method
    NA
  • Shipping Conditions
    NA
  • Storage conditions
    Goat IgG, conjugated with FITC, Isotype control lized at +4ÁC--at least 10 years. Reconstituted at or below -20ÁC--3-5 years reconstituted at +4ÁC--7 days
  • Datasheet link
    NA
  • Description
    Isotype or positive controls by peptides, antibodies and deactivated samples.
  • Properties
    This Exalpha Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Exalpha FITC is highly sensitive to pH extremes. Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.
  • Conjugation
    Anti-FITC Antibody
  • Latin name
    Capra aegagrus hircus
  • About
    Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
  • Group
    isotypes
  • Gene target
  • Short name
    Goat IgG, conjugated with FITC, Isotype control
  • Technique
    Control, Isotype, FITC, Goat, IgG, antibody Conjugates, controls, Fluorescein, goats, IgGs, Isotypes and controls
  • Host
    Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
  • Isotype
    NA, IgG
  • Label
    FITC
  • Species
    Goats
  • Alternative name
    caprine Immunoglobulin G, coupled including fluorecein, isoform reference
  • Alternative technique
    control, controls, caprine, fluorescine, immunoglobulins
  • Tissue
    control
MeSH Data
  • Name
  • Concept
    Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
  • Tree numbers
    • E01.370.225.500.386
    • E05.196.712.516.600.240
    • E05.200.500.386
    • E05.242.386
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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