Alkaline Sphingomyelinase Activity Assay Kit (Colorimetric)

• Catalog number
  K987-100
• Price
  Please ask
• Size
  100 assays

• Description
  Sensitive Assay, HTS
• Summary
  • Detection method- Absorbance (OD 570 nm) • Applications- - Measurement of AlkSMase activity in various tissues/cells extracts - Screening of AlkSMase inhibitors
• Detection Method
  Absorbance (OD 570 nm)
• Applications
  • Sensitive assays for measuring IDH activity in various biological samples and the kit is also suitable for high throughput analyses.
• Sample Type
  Animal tissues: brain, heart, kidney, etc. • Cell culture: Adherent or suspension cells • Characterization of AlkSMase activity of purified recombinant enzyme
• Features Benefits
  • Simple, rapid & convenient • The assay can detect sphingomyleinase activity as low as 0.5 in a variety of samples
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  I. Sphingomyelinase (SMase, E.C. 3.1.4.12) cleaves sphingomyelin to produce phosphocholine and ceramide. Both of these molecules can act as second messengers and regulate downstream events including apoptosis, cell proliferation, and sterol homeostasis. The sphingomyelinase family of enzymes includes acid sphingomyelinase, found in the lysosome and also secreted, neutral sphingomyelinase, which is enriched in the brain, and alkaline sphingomyelinase (AlkSMase), which acts in the intestinal tract. Unlike the neutral variety (nSMase), AlkSMase shows optimal activity at higher pH and does not require mg2+ for enzymatic activity. AlkSMase, also referred to as NPP7, because of its homology to the Nucleotide Pyrophosphatase/Phosphodiesterase family of enzymes, has been implicated in prevention of tumor formation. Its activity is decreased in some intestinal cancers, as wells as instances of chronic colitis. Alkaline Sphingomyelinase may also play a role in cholesterol absorption. BioVision’s Alkaline Sphingomyelinse Colorimetric Assay Kit provides a simple, high throughput adaptable means to identify and quantify alkaline sphingomyelinase activity in a variety of samples without influence from neutral or acid sphingomyelinase. Upon incubation with substrate, the hydrolysis produces an intermediate that reacts with the probe, generating a colorimetric signal. The assay can detect as low as 0.5 µU of alkaline sphingomyelinase activity
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Properties
  Colorimetric assays or detection use UV absorption or enzymatic color reaction.

• Gene target
  Alkaline   Sphingomyelinase   Activity   Kit  
• Short name
  Alkaline Sphingomyelinase Activity Assay Kit (Colorimetric)
• Technique
  colorimetric, Assay, assays, colorimetry
• Host
  Assay
• Alternative name
  Alkaline Sphingomyelinase Activity test reagent (colorimetry)
• Alternative technique
  assays, arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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