Alkaline Phosphatase Activity Fluorometric Assay Kit
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Catalog number
K422-500
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Price
Please ask
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Size
500 assays
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Description
Highly Sensitive Assay, HTS
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Summary
• Detection method- Fluorescence (360/440) • Species reactivity- Mammalian • Applications- The assay designed to measure ALP activity in serum and bio-samples with detection sensitivity ~1 µU
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Sample Type
Cell and tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids
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Features Benefits
• Simple procedure; takes less than 1 hour • Fast and convenient • The kit is more sensitive than colorimetric assays. The kit is suitable for both research and drug discovery.
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Storage Conditions
-20°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
Alkaline phosphatase (ALP) catalyzes the hydrolysis of phosphate esters in alkaline buffer and produces an organic radical and inorganic phosphate. The change in alkaline phosphatase level and activity is associated with a lot of diseases in the liver and bones. Alkaline phosphatase is also a popular enzyme conjugated to secondary antibody in ELISA. In BioVision’s Alkaline Phosphatase Fluorimetric Assay Kit, ALP cleaves the phosphate group of the non-fluorescent 4-Methylumbelliferyl phosphate disodium salt (MUP) substrate resulting in an intense fluorescent signal (Ex/Em = 360nm/440nm). The kit is an ultra sensitive, simple, direct and HTS-ready assay designed to measure ALP activity in serum and bio-samples with detection sensitivity ~1 µU, more sensitive than colorimetric assays. The kit is suitable for both research and drug discovery.
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Test
Biovision supplies other types of Assays as 1.
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Gene target
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Short name
Alkaline Phosphatase Activity Fluorometric Assay Kit
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Technique
Assay, assays
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Host
Assay
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Label
Alkaline Phosphatase
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Alternative name
Alkaline Phosphatase Activity fluorescence test reagent
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Alternative technique
arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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