Rat BACE2(Beta-Site APP Cleaving Enzyme 2) EIA Kit
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Catalog numberE-EL-R0103-96T
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PricePlease ask
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Size96Test
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Detection TypeColormetric
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Target SpeciesRat
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Target SynonymAEPLC, ALP56, ASP1, ASP21, BAE2, CEAP1, DRAP, Beta-secretase 2
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Test principleThis EIA kit uses the Sandwich-EIA principle. The micro EIA plate provided in this kit has been pre-coated with an antibody specific to Rat BACE2. Standards or samples are added to the micro EIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat BACE2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat BACE2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat BACE2. You can calculate the concentration of Rat BACE2 in the samples by comparing the OD of the samples to the standard curve.
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Sensitivity1.88ng/mL
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Detection Range3.13~200ng/mL
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Target NameBACE2
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UNIProt IDQ6IE75
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Tested Sample TypesSerum, Plasma, Cell supernatant
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Product NameRat BACE2(Beta-Site APP Cleaving Enzyme 2) EIA Kit
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TypeSandwich
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DescriptionThis ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C. Enzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.
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PropertiesMeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay
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AboutRats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin nameRattus norvegicus
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Gene target
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Gene symbolAPP-DT, APP, PABPC4, BACE2-IT1, NAE1, BACE2
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Short nameBACE2(Beta-Site APP Cleaving Enzyme 2) EIA Kit
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TechniqueEnzyme, EIA, eia, enzymes
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HostRat
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SpeciesRat, Rats
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Alternative nameRat BACE2(b-Site amyloid beta (A4) precursor protein Cleaving Enzyme 2) EIA reagent
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Alternative techniqueenzymes, kits, elisas
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Alternative to gene targetamyloid beta (A4) precursor protein, AAA and ABETA and ABPP and AD1 and APPI and CTFgamma and CVAP and PN-II and PN2, APP and IDBG-1100 and ENSG00000142192 and 351, growth factor receptor binding, nuclei, App and IDBG-172954 and ENSMUSG00000022892 and 11820, APP and IDBG-630293 and ENSBTAG00000017753 and 280722
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Gene info
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Identity
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Gene
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Long gene nameAPP divergent transcript
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Locus
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Discovery year2020-06-12
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Classification
- Divergent transcripts
Gene info
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Identity
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Gene
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Long gene nameamyloid beta precursor protein
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Synonyms gene
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Synonyms gene name
- Alzheimer disease
- amyloid beta (A4) precursor protein
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Receptor ligands
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namepoly(A) binding protein cytoplasmic 4
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Synonyms gene name
- poly(A)-binding protein, cytoplasmic 4 (inducible form)
- poly(A) binding protein, cytoplasmic 4 (inducible form)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1999-12-17
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- RNA binding motif containing
- Small nucleolar RNA protein coding host genes
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameBACE2 intronic transcript 1
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Synonyms gene
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Synonyms gene name
- chromosome 21 open reading frame 75
- non-protein coding RNA 228
- BACE2 intronic transcript 1 (non-protein coding)
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Synonyms
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Locus
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Discovery year2002-11-26
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Entrez gene record
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Classification
- Intronic transcripts
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameNEDD8 activating enzyme E1 subunit 1
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Synonyms gene
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Synonyms gene name
- amyloid beta precursor protein binding protein 1, 59kDa
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-01-13
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Ubiquitin like modifier activating enzymes
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namebeta-secretase 2
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Synonyms gene
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Synonyms gene name
- beta-site APP-cleaving enzyme 2
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2000-05-23
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Entrez gene record
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Pubmed identfication
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Classification
- Peptidase family A1
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
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Tree numbers
- E05.478.566.350
- E05.478.583.400
- E05.601.470.350
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data