Rabbit Anti-Rat UT-A1 Polyclonal: ATTO 633
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Catalog numberSPC-406D-A633
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Price:
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Size100 µg
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ImmunogenProduced against a synthetic peptide mapped to the C-terminal tail (amino acids 911-929) of rat UT-A1 (antibody designation L194)
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Antibody s targetRat UT-A1
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Antibody s full descriptionRabbit Anti-Rat UT-A1 Polyclonal
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Primary research fieldsNeuroscience, Pumps/Transporters, Urea Transporters
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Antibody s categoryPolyclonal Antibodies
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Antibody s other nameSLC14A2 Antibody, FLJ16167 Antibody, hUT-A6 Antibody, HUT2 Antibody, kidney Antibody, MGC119566 Antibody, MGC119567 Antibody, Slc14a2 Antibody, Solute carrier family 14 (urea transporter) Antibody, member 2 Antibody, Solute carrier family 14 member 2 Antibody, Urea transporter 2 Antibody, Urea transporter Antibody, Urea transporter kidney Antibody, UT-A2 Antibody, UT2 Antibody, UT2_HUMAN Antibody, UTA Antibody, UTR Antibody, UT1 Antibody, UTA1 Antibody
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Verified applicationsWB, ICC/IF
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Raised inRabbit
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Antibody s reactivityRat, Mouse
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Antibody s recommended dilutions for useWB (1:1000); optimal dilutions for assays should be determined by the user.
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Antibody s purified fromProtein A Purified
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Recommended buffer for storagePBS, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~97 and 127kDa.
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Storage recommendations-20°C
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Shipping recommendationsBlue Ice or 4°C
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Certificate of analysis1 µg/ml of SPC-406 was sufficient for detection of UT-A1 in 20 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cellCell Membrane, Apical Cell Membrane
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Tissue specificitySee included datasheet or contact our support service.
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Scientific contextUT-A1, a kidney-specific urea transporter is expressed in the renal collecting duct where it mediates trans-epithelial urea transport and is a target for regulation by vasopressin. Urea movement out of the collecting duct in the inner medulla of the kidney allows accumulation of urea in the medullary interstitium, thereby allowing maximum water reabsorption from the collecting ducts. (The antibody also recognizes a second protein from the UT-A gene driven by transcription from an alternative promoter and expressed in the thin descending limb of Henle, viz. UT-A2) (1, 2).
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Bibliography1. Nielsen S., et al. (1996) Proc Natl Acad Sci U S A. 93(11): 5495-500. 2. Smith C.P. (2009) Exp Physiol. 94(2): 180-185.
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Released date11/Jun/2010
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NCBI numberNP_062220
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Gene number54302
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Protein numberQ62668
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PubMed numberRefer to PubMed
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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Antibody s datasheetContact our support service to receive datasheet or other technical documentation.
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Representative figure legendWestern blot analysis of Rat Inner medulla showing detection of UT-A1 protein using Rabbit Anti-UT-A1 Polyclonal Antibody (SPC-406). Primary Antibody: Rabbit Anti-UT-A1 Polyclonal Antibody (SPC-406) at 1:1000. Rabbit Anti-UT-A1 Antibody used in Western blot (WB) on Rat Inner medulla (SPC-406)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilitIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin nameOryctolagus cuniculus, Rattus norvegicus
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Gene target
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Gene symbolRFC4, RFC2, SNAR-A1, PCDHGA1, ZNF2, SCAF1, FALEC, HNRNPA1, RFC1, EDA
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Short nameRabbit Anti- UT-A1 Polyclonal: ATTO 633
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TechniquePolyclonal, Rabbit, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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HostRabbit, Rabbits
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IsotypeImmunoglobulin
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LabelATTO 633
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SpeciesRat, Rats
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Alternative nameproduction species: rabbit Antibody toRat UT-A1 polyclonal: ATTO 633
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Alternative techniquepolyclonals, rabbit-anti, antibodies
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Gene info
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Identity
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Gene
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Long gene namereplication factor C subunit 4
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Synonyms gene name
- replication factor C (activator 1) 4 (37kD)
- replication factor C (activator 1) 4, 37kDa
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Synonyms
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Synonyms name
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Locus
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Discovery year1994-10-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- AAA ATPases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namereplication factor C subunit 2
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Synonyms gene name
- replication factor C (activator 1) 2 (40kD)
- replication factor C (activator 1) 2, 40kDa
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Synonyms
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Synonyms name
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Locus
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Discovery year1994-10-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- AAA ATPases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesmall NF90 (ILF3) associated RNA A1
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Synonyms gene name
- small nuclear ILF3/NF90-associated RNA A1
- small ILF3/NF90-associated RNA A1
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-06-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small NF90 (ILF3) associated RNAs
Gene info
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Identity
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Gene
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Long gene nameprotocadherin gamma subfamily A, 1
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-06-28
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Clustered protocadherins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namezinc finger protein 2
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Synonyms gene name
- zinc finger protein 2 (A1-5)
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Synonyms
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GenBank acession
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Locus
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Discovery year1989-05-31
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Zinc fingers C2H2-type
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameSR-related CTD associated factor 1
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-08-09
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namefocally amplified long non-coding RNA in epithelial cancer
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Synonyms gene
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Synonyms gene name
- long intergenic non-protein coding RNA 568
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2012-02-02
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Long non-coding RNAs with non-systematic symbols
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameheterogeneous nuclear ribonucleoprotein A1
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Synonyms gene
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Synonyms
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GenBank acession
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Locus
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Discovery year1991-05-21
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- RNA binding motif containing
- Spliceosomal A complex
- Heterogeneous nuclear ribonucleoproteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namereplication factor C subunit 1
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Synonyms gene name
- replication factor C (activator 1) 1 (145kD)
- replication factor C (activator 1) 1, 145kDa
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Synonyms
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GenBank acession
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Locus
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Discovery year1994-10-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- AAA ATPases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameectodysplasin A
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Synonyms gene
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Synonyms gene name
- ectodermal dysplasia 1, anhidrotic
- oligodontia 1
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- MicroRNA protein coding host genes
- Tumor necrosis factor superfamily
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data