- Catalog numberSPC-161F-A633
- Product nameRabbit Anti-Phosphotyrosine Polyclonal: ATTO 633
- Size400 µl
- PriceAsk For Price
- LabelATTO 633
- ImmunogenPhosphotyrosine conjugated to KLH
- Antibody s targetPhosphotyrosine
- Antibody s full descriptionRabbit Anti-Phosphotyrosine Polyclonal
- Primary research fieldsCell Signaling, Post-Translational Modifications, Phosphorylation
- Antibody s categoryPolyclonal Antibodies
- Antibody s other namePhospho-tyrosine Antibody
- Verified applicationsWB, IHC, ICC/IF, IP, ELISA
- Raised inRabbit
- IsotypeImmunoglobulin
- Antibody s reactivitySpecies Independent
- Antibody s recommended dilutions for useWB (1:500), IHC (1:100), ICC/IF (1:50), ELISA (1:2000), IP (1:100); optimal dilutions for assays should be determined by the user.
- Antibody s purified fromProtein A Purified
- Recommended buffer for storagePBS pH7.0, 0.01% sodium azide
- Antibody s concentration0.25 mg/ml
- Antibody s specificityDetects proteins phosphorylated on tyrosine residues. Does not cross-react with phosphoserine or threonine.
- Storage recommendations4°C
- Shipping recommendationsBlue Ice or 4°C
- Certificate of analysisA 1:250 dilution of SPC-161 was sufficient for detection of tyrosine-phosphorylated species in mouse spleen lysates in western blot analysis.
- Antibody in cellContact our support service.
- Tissue specificitySee included datasheet or contact our support service.
- Scientific contextProtein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein’s activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine; others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time (1). Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (2-4). In particular, the phosphorylation of tyrosine is considered one of the key steps in signal transduction and regulation of enzymatic activity (5). Phosphotyrosine can be detected through specific antibodies, and are helpful in facilitating the identification of tyrosine kinase substrates (6).
- Bibliography1. Goto H. et al. (2005) Nature Cell Biology 8: 180-187. 2. Blume-Jensen P. and Hunter T. (2001) Nature 411: 355-365. 3. Downward J. (2001) Nature 411: 759-762. 4. Pawson T. and Saxton T.M. (1999) Cell 97: 675-678. 5. Frackelton A.R. Jr., Ross A.H., and Eisen H.N. (1983) Mol Cell Biol. 3: 1343-1352. 6. Ross A.H., Baltimore D., and Eisen H.N. (1981) Nature 294: 654-656. 7. Ostrovsky PC. (1995) Genes Dev. 9(16): 2034-2041.
- Released date1/Jun/2007
- NCBI numberRefer to NCBI
- Gene numberRefer to GenBank
- Protein numberRefer to Swiss-Prot
- PubMed numberRefer to PubMed
- Tested applicationsTo be tested
- Tested reactivityTo be tested
- Antibody s datasheetContact our support service to receive datasheet or other technical documentation.
- Representative figure link
- Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Phosphotyrosine Polyclonal Antibody (SPC-161). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Phosphotyrosine Polyclonal Antibody (SPC-161) at 1:50 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Nucleus. Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Phosphotyrosine Antibody. (C) Composite. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Phosphotyrosine Polyclonal Antibody (SPC-161). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Phosphotyrosine Polyclonal Antibody (SPC-161) at 1:50 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Nucleus. Cytoplasm. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Phosphotyrosine Antibody. (C) Composite. | Immunohistochemistry analysis using Rabbit Anti-Phosphotyrosine Polyclonal Antibody (SPC-161). Tissue: breast cancer. Species: Human . Primary Antibody: Rabbit Anti-Phosphotyrosine Polyclonal Antibody (SPC-161) at 1:100. Rabbit Anti-Phosphotyrosine Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SPC-161) | Rabbit Anti-Phosphotyrosine Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SPC-161) | Rabbit Anti-Phosphotyrosine Antibody used in Immunohistochemistry (IHC) on Human breast cancer (SPC-161)
- Warning informationNon-hazardous
- Country of productionCanada
- Total weight kg1.4
- Net weight g0.4
- Stock availabilitIn Stock
- Other related products
- Gene targetPhosphotyrosine ATTO 633
- HostRabbit, Rabbits
- isotype filter
- Immunoglobulin
- Short nameRabbit Anti-Phosphotyrosine Polyclonal: ATTO 633
- label filter
- ATTO 633
- technique filter
- Polyclonal
- Rabbit
- anti-
- anti
- TechniquePolyclonal, Rabbit, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
- Alternative techniquepolyclonals, rabbit-anti, antibodies