-
Immunogen
Synthetic peptide of human Ret (AA177-191)
-
Antibody s target
Human RET
-
Antibody s full description
Rabbit Anti-Human RET Polyclonal
-
Antibody s category
Polyclonal Antibodies
-
Antibody s other name
C ret antibody, Cadherin family member 12 antibody, Cadherin related family member 16 antibody, CDHF 12 antibody, CDHF12 antibody, CDHR16 antibody, HSCR 1 antibody, Hydroxyaryl protein kinase antibody, MEN2A antibody, MEN2B antibody, MTC 1 antibody, MTC1 antibody, Multiple endocrine neoplasia and medullary thyroid carcinoma 1 antibody, Oncogene RET antibody, PTC antibody, RET antibody, RET ELE1 antibody, Ret Proto oncogene antibody, RET transforming sequence antibody, RET_HUMAN antibody, RET51 antibody, RET9 antibody, tyrosine-protein kinase receptor ret antibody
-
Verified applications
WB, AM
-
Raised in
Rabbit
-
Antibody s reactivity
Human
-
Antibody s recommended dilutions for use
WB (1:250); optimal dilutions for assays should be determined by the user.
-
Antibody s purified from
Peptide Affinity Purified
-
Recommended buffer for storage
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
-
Antibody s concentration
See included datasheet or contact our support service
-
Antibody s specificity
Detects 124.319 kDa.
-
Storage recommendations
-20°C
-
Shipping recommendations
Blue Ice or 4°C
-
Certificate of analysis
A 1:250 dilution of SPC-1196 was sufficient for detection of RET in 10 µg of human A549 cell line lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
-
Antibody in cell
Cell Membrane
-
Tissue specificity
See included datasheet or contact our support service.
-
Scientific context
RET is a receptor-tyrosine kinase that is activated upon binding glial-derived neurotrophic factor (GDNF). Normally involved in the differentiation of neural crest cells and organs (salivary glands, spleen, thyroid, thymus etc). Given its vary clearly identified role in thyroid carcinoma and also the known oncogenic potential of RTKs, it is not surprising that this gene is characterized as a proto-oncogene. Fusion partners result in disregulated kinase activity related both to constituent activation and promiscuous protein interaction.
-
Bibliography
Refer to PubMed
-
Released date
29/Aug/2016
-
NCBI number
NP_066124
-
Gene number
5979
-
Protein number
P07949
-
PubMed number
Refer to PubMed
-
Tested applications
To be tested
-
Tested reactivity
To be tested
-
Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
-
Representative figure link
No Data Available
-
Warning information
Non-hazardous
-
Country of production
Canada
-
Total weight kg
1.4
-
Net weight g
0.1
-
Stock availabilit
In Stock
-
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
Test
StressMark antibodies supplies antibodies that are for research of human proteins.
-
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Group
Polyclonals and antibodies
-
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
-
Latin name
Oryctolagus cuniculus
-
Gene target
-
Gene symbol
GOLGA5, RET
-
Short name
Rabbit Anti- RET Polyclonal: Streptavidin
-
Technique
anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
-
Host
Rabbit, Rabbits
-
Isotype
Immunoglobulin
-
Label
Streptavidin
-
Species
Human, Humans
-
Alternative name
production species: rabbit Antibody toHuman ret proto-oncogene polyclonal: biotinilated-binding streptavidin
-
Alternative technique
polyclonals, rabbit-anti, antibodies
-
Alternative to gene target
ret proto-oncogene, CDHF12 and CDHR16 and HSCR1 and MEN2A and MEN2B and MTC1 and PTC and RET-ELE1 and RET51, RET and IDBG-71064 and ENSG00000165731 and 5979, transferase activity, Plasma membranes, Ret and IDBG-181393 and ENSMUSG00000030110 and 19713, RET and IDBG-628722 and ENSBTAG00000000570 and 515924
-
