Rabbit Anti-Human PCNA Polyclonal: ATTO 488
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Catalog numberSPC-1307D-A488
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Price:
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Size100 µl
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ImmunogenSynthesized peptide derived from human PCNA.
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Antibody s targetHuman PCNA
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Antibody s full descriptionRabbit Anti-Human PCNA Polyclonal
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Antibody s categoryPolyclonal Antibodies
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Antibody s other nameATLD2 Antibody, cb16 Antibody, Cyclin Antibody, DNA polymerase delta auxiliary protein Antibody, etID36690.10 Antibody, fa28e03 Antibody, fb36g03 Antibody, HGCN8729 Antibody, MGC8367 Antibody, Mutagen-sensitive 209 protein Antibody, OTTHUMP00000030189 Antibody, OTTHUMP00000030190 Antibody, PCNA Antibody, Pcna/cyclin Antibody, PCNA_HUMAN Antibody, PCNAR Antibody, Polymerase delta accessory protein Antibody, Proliferating cell nuclear antigen Antibody, wu:fa28e03 Antibody, wu:fb36g03 Antibodya
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Verified applicationsWB, IHC, ELISA
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Raised inRabbit
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Antibody s reactivityHuman, Mouse, Rat
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Antibody s recommended dilutions for useWB (1:1000), IHC (1:100), ELISA (1:5000); optimal dilutions for assays should be determined by the user.
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Antibody s purified fromPeptide Affinity Purified
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Recommended buffer for storagePBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
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Antibody s concentrationSee included datasheet or contact our support service
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Antibody s specificityDetects ~29kDa, endogenous levels of total PCNA protein.
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Storage recommendations-20°C
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Shipping recommendationsBlue Ice or 4°C
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Certificate of analysisA 1:1000 dilution of SPC-1307 was sufficient for detection of PCNA in 10 µg of HepG2 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
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Antibody in cellNucleus
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Tissue specificitySee included datasheet or contact our support service.
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Scientific contextProliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase δ in eukaryotic cells and is essential for replication. It is a 36 kDa molecule that is highly conserved between species. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand.
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Bibliography1. Moldovan GL. et al. (2007) Cell 129 (4): 665–79. 2. Warbrick E. et al. (1998) BioEssays 20 (3): 195–9. 3. Gilljam KM. et al. (2009) The Journal of Cell Biology 186 (5): 645–54.
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Released date6/Sep/2016
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NCBI numberNP_872590.1
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Gene number5111
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Protein numberP12004
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PubMed numberRefer to PubMed
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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Antibody s datasheetContact our support service to receive datasheet or other technical documentation.
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Representative figure link
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Representative figure legendImmunohistochemistry analysis using Rabbit Anti-PCNA Polyclonal Antibody (SPC-1307). Tissue: Breast Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-PCNA Polyclonal Antibody (SPC-1307) at 1:100. The image on the right is treated with the synthesized peptide. | Western blot analysis of Human HepG2 cell lysates showing detection of ~29kDa PCNA protein using Rabbit Anti-PCNA Polyclonal Antibody (SPC-1307). Lane 1: Human HepG2 cells. Lane 2: Human HepG2 cells treated with the immunizing peptide. Primary Antibody: Rabbit Anti-PCNA Polyclonal Antibody (SPC-1307) at 1:1000. Predicted/Observed Size: ~29kDa. Rabbit Anti-PCNA Antibody used in Immunohistochemistry (IHC) on Breast Carcinoma Tissue (SPC-1307) | Rabbit Anti-PCNA Antibody used in Western blot (WB) on HepG2 cell lysates (SPC-1307)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilitIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestStressMark antibodies supplies antibodies that are for research of human proteins.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin nameOryctolagus cuniculus
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Gene target
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Gene symbolPCNA, PCNA-AS1
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Short nameRabbit Anti- PCNA Polyclonal: ATTO 488
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Techniqueanti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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HostRabbit, Rabbits
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IsotypeIgG
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LabelATTO 488
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SpeciesHuman, Humans
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Alternative nameproduction species: rabbit Antibody toHuman PCNA polyclonal: ATTO 488
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Alternative techniquepolyclonals, rabbit-anti, antibodies
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Gene info
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Identity
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Gene
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Long gene nameproliferating cell nuclear antigen
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GenBank acession
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Locus
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Discovery year1990-01-15
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Entrez gene record
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Pubmed identfication
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namePCNA antisense RNA 1
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Synonyms gene
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Synonyms gene name
- PCNA antisense RNA (non-protein coding)
- PCNA antisense RNA 1 (non-protein coding)
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Locus
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Discovery year2009-08-18
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Antisense RNAs
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data