- Catalog numberSPC-691D-A565
- Product nameRabbit Anti-Human PCNA Polyclonal: ATTO 565
- Size100 µg
- PriceAsk For Price
- LabelATTO 565
- ImmunogenSynthetic peptide of Human PCNA
- Antibody s targetHuman PCNA
- Antibody s full descriptionRabbit Anti-Human PCNA Polyclonal
- Primary research fieldsCancer, Tumor Biomarkers, Tumor Antigens, Cell Signaling, Epigenetics and Nuclear Signalling, DNA/RNA, DNA Synthesis
- Antibody s categoryPolyclonal Antibodies
- Antibody s other namecb16 Antibody, MGC8367 Antibody, PCNA_HUMAN Antibody, Polymerase delta accessory protein Antibody, DNA polymerase delta auxiliary protein Antibody, Proliferating cell nuclear antigen Antibody, Mutagen-sensitive 209 protein Antibody, OTTHUMP00000030189 Antibody, PCNAR Antibody, Pcna/cyclin Antibody, HGCN8729 Antibody, wu:fb36g03 Antibody, PCNA Antibody, ATLD2 Antibody, fb36g03 Antibody, OTTHUMP00000030190 Antibody, Cyclin Antibody
- Verified applicationsWB
- Raised inRabbit
- IsotypeImmunoglobulin
- Antibody s reactivityHuman
- Antibody s recommended dilutions for useWB (1:1000); optimal dilutions for assays should be determined by the user.
- Antibody s purified fromPeptide Affinity Purified
- Recommended buffer for storagePBS, 50% glycerol, 0.09% sodium azide
- Antibody s concentration1 mg/ml
- Antibody s specificityDetects ~37 kDa.
- Storage recommendations-20°C
- Shipping recommendationsBlue Ice or 4°C
- Certificate of analysisA 1:1000 dilution of SPC-691 was sufficient for detection of PCNA in 15 µg of Human 293T Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
- Antibody in cellNucleus
- Scientific contextSee included datasheet or contact our support service.
- BibliographyRefer to PubMed
- Released date29/Aug/2016
- NCBI numberNP_002583.1
- Gene number5111
- Protein numberP12004
- PubMed numberRefer to PubMed
- Tested applicationsTo be tested
- Tested reactivityTo be tested
- Antibody s datasheetContact our support service to receive datasheet or other technical documentation.
- Representative figure link
- Representative figure legendWestern blot analysis of Human 293 Trap cell lysates showing detection of ~28.7 kDa PCNA protein using Rabbit Anti-PCNA Polyclonal Antibody (SPC-691). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human 293Trap cell lysates. Load: 15 µg . Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-PCNA Polyclonal Antibody (SPC-691) at 1:1000 for 1 hour. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 1 hour RT with shaking. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~28.7 kDa. Rabbit Anti-PCNA Antibody used in Western blot (WB) on 293 Trap cell lysates (SPC-691)
- Warning informationNon-hazardous
- Country of productionCanada
- Total weight kg1.4
- Net weight g0.1
- Stock availabilitIn Stock
- Other related products
- Gene targetPCNA ATTO 565
- Identity:HGNC:8729
- Gene:PCNA
- Long gene name:proliferating cell nuclear antigen
- Discovery year:1990-01-15
- Entrez gene record:5111
- Pubmed identification:2565339
- VEGA ID:OTTHUMG00000031798
- Identity:HGNC:37184
- Gene:PCNA-AS1
- Long gene name:PCNA antisense RNA 1
- Synonyms gene name:PCNA antisense RNA (non-protein coding), PCNA antisense RNA 1 (non-protein coding)
- Discovery year:2009-08-18
- Entrez gene record:100302739
- Pubmed identification:10488129, 24704293
- RefSeq identity:NR_028370
- Classification:Antisense RNAs
- Gene symbolPCNA, PCNA-AS1
- HostRabbit, Rabbits
- isotype filter
- Immunoglobulin
- Short nameRabbit Anti- PCNA Polyclonal: ATTO 565
- label filter
- ATTO 565
- technique filter
- anti-Human
- Polyclonal
- Rabbit
- anti-
- anti
- Techniqueanti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
- Alternative techniquepolyclonals, rabbit-anti, antibodies