-
Immunogen
Human p90 RSK1 C-terminal peptide, conjugated to KLH
-
Antibody s target
Human RSK1
-
Antibody s full description
Rabbit Anti-Human p90 RSK1 Polyclonal
-
Primary research fields
Cell Signaling, Post-translational modifications, Phosphorylation
-
Antibody s category
Polyclonal Antibodies
-
Antibody s other name
RSK1 p90 Antibody, HU1 Antibody, MAPKAP Antibody, p90rsk Antibody, p90S6K Antibody, RSK1 Antibody, RSK 1 p90 Antibody
-
Verified applications
WB, ICC/IF, IP, ELISA
-
Raised in
Rabbit
-
Antibody s reactivity
Human, Mouse, Rat, Bovine
-
Antibody s recommended dilutions for use
WB (1:1000), ICC/IF (1:50); optimal dilutions for assays should be determined by the user.
-
Antibody s purified from
Protein A Purified
-
Recommended buffer for storage
TBS, 50% glycerol, 0.09% sodium azide
-
Antibody s concentration
0.25 mg/ml
-
Antibody s specificity
Detects ~90kDa.
-
Storage recommendations
-20°C
-
Shipping recommendations
Blue Ice or 4°C
-
Certificate of analysis
0.25 mg/ml was sufficient for detection of SPC-147 in lysates prepared from mouse brain, spleen and intestine.
-
Antibody in cell
Cytoplasm, Nucleus
-
Tissue specificity
See included datasheet or contact our support service.
-
Scientific context
The p90 ribosomal S6 kinase (Rsk) family comprises four mammalian serine/threonine kinases (Rsk1–4). In the past, S6K1 and S6K2 were thought to be the predominant operating S6 kinases, however RSK 1 and 2 have been shown to phosphorylate S6 in response to the ERK pathway, or otherwise known as the extra cellular signal-regulated kinases pathway. RSK 1 in particular is very multifunctional as it participates in nuclear signaling, regulates nuclear factors, regulates several transcription factors like c-Fos, and interacts with the transcriptional coactivator CREB-binding protein. As a result, RSK1 seems to have an important role in cellular growth control and proliferation. (1-5).
-
Bibliography
1. Roux, P. (2007). UCSD-Nature Molecule Pages. 2. Fro¨din, M. and Gammeltoft, S. (1999) Mol. Cell. Endocrinol. 151: 65-77. 3. Merienne, K. et al. (1998) J. Med. Genet. 35: 890-894. 4. De Cesare, D. et al. (1998) Proc. Natl. Acad. Sci. 95: 12202-12207. 5. Joel, P. B. et al. (1998) Mol. Cell. Biol. 18: 1978-1984.
-
Released date
1/Jun/2007
-
NCBI number
NP_002944.2
-
Gene number
6195
-
Protein number
Q15418
-
PubMed number
Refer to PubMed
-
Tested applications
To be tested
-
Tested reactivity
To be tested
-
Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
-
Representative figure link
-
Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-p90 RSK1 Polyclonal Antibody (SPC-147). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-p90 RSK1 Polyclonal Antibody (SPC-147) at 1:50 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Nucleus. Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-p90 RSK1 Antibody. (C) Composite. Heat Shocked at 42°C for 30 min. | Western blot analysis of Mouse brain cell lysates showing detection of RSK1 protein using Rabbit Anti-RSK1 Polyclonal Antibody (SPC-147). Primary Antibody: Rabbit Anti-RSK1 Polyclonal Antibody (SPC-147) at 1:1000. P90 RSK1 immuno-precipitated from the mouse brain extract (left) and immuno-precipitated negative control (right). | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-p90 RSK1 Polyclonal Antibody (SPC-147). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-p90 RSK1 Polyclonal Antibody (SPC-147) at 1:50 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Nucleus. Cytoplasm. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-p90 RSK1 Antibody. (C) Composite. Heat Shocked at 42°C for 30 min. Rabbit Anti-p90 RSK1 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SPC-147) | Rabbit Anti-RSK1 Antibody used in Western blot (WB) on Mouse brain cell lysates (SPC-147) | Rabbit Anti-p90 RSK1 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SPC-147)
-
Warning information
Non-hazardous
-
Country of production
Canada
-
Total weight kg
1.4
-
Net weight g
0.4
-
Stock availabilit
In Stock
-
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
Test
StressMark antibodies supplies antibodies that are for research of human proteins.
-
Properties
This StressMark antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. StressMark antibodies FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Conjugation
Anti-FITC Antibody
-
Group
Polyclonals and antibodies
-
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
-
Latin name
Oryctolagus cuniculus
-
Gene target
-
Gene symbol
RPS6KA1
-
Short name
Rabbit Anti- p90 RSK1 Polyclonal: FITC
-
Technique
anti-Human, Polyclonal, Rabbit, anti-, anti, FITC, antibody to, antibodies, antibodies against human proteins, Fluorescein, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
-
Host
Rabbit, Rabbits
-
Isotype
Immunoglobulin
-
Label
FITC
-
Species
Human, Humans
-
Alternative name
production species: rabbit Antibody toHuman p90 RSK1 polyclonal: fluorecein
-
Alternative technique
polyclonals, rabbit-anti, antibodies, fluorescine
-
