Rabbit Anti-Human p90 RSK1 Polyclonal: ATTO 655

  • Catalog number
    SPC-147F-A655
  • Price
    Please ask
  • Size
    400 µl
  • Immunogen
    Human p90 RSK1 C-terminal peptide, conjugated to KLH
  • Antibody s target
    Human RSK1
  • Antibody s full description
    Rabbit Anti-Human p90 RSK1 Polyclonal
  • Primary research fields
    Cell Signaling, Post-translational modifications, Phosphorylation
  • Antibody s category
    Polyclonal Antibodies
  • Antibody s other name
    RSK1 p90 Antibody, HU1 Antibody, MAPKAP Antibody, p90rsk Antibody, p90S6K Antibody, RSK1 Antibody, RSK 1 p90 Antibody
  • Verified applications
    WB, ICC/IF, IP, ELISA
  • Raised in
    Rabbit
  • Antibody s reactivity
    Human, Mouse, Rat, Bovine
  • Antibody s recommended dilutions for use
    WB (1:1000), ICC/IF (1:50); optimal dilutions for assays should be determined by the user.
  • Antibody s purified from
    Protein A Purified
  • Recommended buffer for storage
    TBS, 50% glycerol, 0.09% sodium azide
  • Antibody s concentration
    0.25 mg/ml
  • Antibody s specificity
    Detects ~90kDa.
  • Storage recommendations
    -20°C
  • Shipping recommendations
    Blue Ice or 4°C
  • Certificate of analysis
    0.25 mg/ml was sufficient for detection of SPC-147 in lysates prepared from mouse brain, spleen and intestine.
  • Antibody in cell
    Cytoplasm, Nucleus
  • Tissue specificity
    See included datasheet or contact our support service.
  • Scientific context
    The p90 ribosomal S6 kinase (Rsk) family comprises four mammalian serine/threonine kinases (Rsk1–4). In the past, S6K1 and S6K2 were thought to be the predominant operating S6 kinases, however RSK 1 and 2 have been shown to phosphorylate S6 in response to the ERK pathway, or otherwise known as the extra cellular signal-regulated kinases pathway. RSK 1 in particular is very multifunctional as it participates in nuclear signaling, regulates nuclear factors, regulates several transcription factors like c-Fos, and interacts with the transcriptional coactivator CREB-binding protein. As a result, RSK1 seems to have an important role in cellular growth control and proliferation. (1-5).
  • Bibliography
    1. Roux, P. (2007). UCSD-Nature Molecule Pages. 2. Fro¨din, M. and Gammeltoft, S. (1999) Mol. Cell. Endocrinol. 151: 65-77. 3. Merienne, K. et al. (1998) J. Med. Genet. 35: 890-894. 4. De Cesare, D. et al. (1998) Proc. Natl. Acad. Sci. 95: 12202-12207. 5. Joel, P. B. et al. (1998) Mol. Cell. Biol. 18: 1978-1984.
  • Released date
    1/Jun/2007
  • NCBI number
    NP_002944.2
  • Gene number
    6195
  • Protein number
    Q15418
  • PubMed number
    Refer to PubMed
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • Antibody s datasheet
    Contact our support service to receive datasheet or other technical documentation.
  • Representative figure link
  • Representative figure legend
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-p90 RSK1 Polyclonal Antibody (SPC-147). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-p90 RSK1 Polyclonal Antibody (SPC-147) at 1:50 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Nucleus. Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-p90 RSK1 Antibody. (C) Composite. Heat Shocked at 42°C for 30 min. | Western blot analysis of Mouse brain cell lysates showing detection of RSK1 protein using Rabbit Anti-RSK1 Polyclonal Antibody (SPC-147). Primary Antibody: Rabbit Anti-RSK1 Polyclonal Antibody (SPC-147) at 1:1000. P90 RSK1 immuno-precipitated from the mouse brain extract (left) and immuno-precipitated negative control (right). | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-p90 RSK1 Polyclonal Antibody (SPC-147). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-p90 RSK1 Polyclonal Antibody (SPC-147) at 1:50 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Nucleus. Cytoplasm. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-p90 RSK1 Antibody. (C) Composite. Heat Shocked at 42°C for 30 min. Rabbit Anti-p90 RSK1 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SPC-147) | Rabbit Anti-RSK1 Antibody used in Western blot (WB) on Mouse brain cell lysates (SPC-147) | Rabbit Anti-p90 RSK1 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SPC-147)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.4
  • Stock availabilit
    In Stock
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Test
    StressMark antibodies supplies antibodies that are for research of human proteins.
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Group
    Polyclonals and antibodies
  • About
    Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
  • Latin name
    Oryctolagus cuniculus
  • Gene target
    p90   RSK1   ATTO   655  
  • Gene symbol
    RPS6KA1
  • Short name
    Rabbit Anti- p90 RSK1 Polyclonal: ATTO 655
  • Technique
    anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Host
    Rabbit, Rabbits
  • Isotype
    Immunoglobulin
  • Label
    ATTO 655
  • Species
    Human, Humans
  • Alternative name
    production species: rabbit Antibody toHuman p90 RSK1 polyclonal: ATTO 655
  • Alternative technique
    polyclonals, rabbit-anti, antibodies
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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