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Immunogen
Synthesized peptide derived from Internal of human p27 Kip1.
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Antibody s target
Human p27 Kip1
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Antibody s full description
Rabbit Anti-Human p27 Kip1 Polyclonal
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Primary research fields
Cancer, Cell Cycle, Cip/Kip
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
Cdki1b Antibody, CDKN 1B Antibody, CDKN 4 Antibody, Cyclin Dependent Kinase Inhibitor 1B Antibody, Cyclin dependent kinase inhibitor p27 Antibody, Cyclin-dependent kinase inhibitor p27 Kip1 Antibody, KIP 1 Antibody, MEN1B Antibody, MEN4 Antibody, p27 Antibody, p27 Kip1 Antibody
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Verified applications
IHC, ELISA
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Raised in
Rabbit
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Antibody s reactivity
Human
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Antibody s recommended dilutions for use
IHC (1:100), ELISA (1:40000); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects endogenous levels of total p27 Kip1 protein.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:100 dilution of SPC-1282 was sufficient for detection of p27 Kip1 in human brain tissue using Goat Anti-Rabbit IgG as the secondary antibody.
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Antibody in cell
Nucleus, Cytoplasm, Endosome
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Tissue specificity
Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney.
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Scientific context
The cyclin-dependent kinase inhibitor, p27 Kip1 is a tumor suppressor protein that acts in the nucleus to enforce cell cycle checkpoints. Like its relatives, p57 Kip2 and p21 Waf1/Cip1, the ability to enforce the G1 restriction point is derived from its inhibitory binding to CDK2/cyclin E and other CDK/cyclin complexes. Expression levels of p27 are upregulated in quiescent cells and in cells treated with cAMP or other negative cell cycle regulators. Downregulation of p27 can be induced by treatment with interleukin-2 or other mitogens; this involves phosphorylation of p27 and its degradation by the ubiquitin-proteasome pathway (1-4).
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Bibliography
1. Lloyd, R.V. et al. (1999) Am J Pathol 154, 313-23. 2. Polyak, K. et al. (1994) Genes Dev 8, 9-22. 3. Kato, J.Y. et al. (1994) Cell 79, 487-96. 4. Vlach, J. et al. (1997) EMBO J 16, 5334-44.
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Released date
6/Sep/2016
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NCBI number
NP_004055.1
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Gene number
1027
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Protein number
P46527
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure legend
Immunohistochemistry analysis using Rabbit Anti-p27 Kip1 Polyclonal Antibody (SPC-1282). Tissue: Brain tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-p27 Kip1 Polyclonal Antibody (SPC-1282) at 1:100. The image on the right is treated with the synthesized peptide. Rabbit Anti-p27 Kip1 Antibody used in Immunohistochemistry (IHC) on Brain tissue (SPC-1282)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
