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Rabbit Anti-Human p27 Kip1 Polyclonal: ATTO 633

  • Catalog number
    SPC-1282D-A633
  • Product name
    Rabbit Anti-Human p27 Kip1 Polyclonal: ATTO 633
  • Size
    100 µl
  • Label
    ATTO 633
  • Immunogen
    Synthesized peptide derived from Internal of human p27 Kip1.
  • Antibody s target
    Human p27 Kip1
  • Antibody s full description
    Rabbit Anti-Human p27 Kip1 Polyclonal
  • Primary research fields
    Cancer, Cell Cycle, Cip/Kip
  • Antibody s category
    Polyclonal Antibodies
  • Antibody s other name
    Cdki1b Antibody, CDKN 1B Antibody, CDKN 4 Antibody, Cyclin Dependent Kinase Inhibitor 1B Antibody, Cyclin dependent kinase inhibitor p27 Antibody, Cyclin-dependent kinase inhibitor p27 Kip1 Antibody, KIP 1 Antibody, MEN1B Antibody, MEN4 Antibody, p27 Antibody, p27 Kip1 Antibody
  • Verified applications
    IHC, ELISA
  • Raised in
    Rabbit
  • Isotype
    IgG
  • Antibody s reactivity
    Human
  • Antibody s recommended dilutions for use
    IHC (1:100), ELISA (1:40000); optimal dilutions for assays should be determined by the user.
  • Antibody s purified from
    Peptide Affinity Purified
  • Recommended buffer for storage
    PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
  • Antibody s concentration
    See included datasheet or contact our support service
  • Antibody s specificity
    Detects endogenous levels of total p27 Kip1 protein.
  • Storage recommendations
    -20°C
  • Shipping recommendations
    Blue Ice or 4°C
  • Certificate of analysis
    A 1:100 dilution of SPC-1282 was sufficient for detection of p27 Kip1 in human brain tissue using Goat Anti-Rabbit IgG as the secondary antibody.
  • Antibody in cell
    Nucleus, Cytoplasm, Endosome
  • Tissue specificity
    Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney.
  • Scientific context
    The cyclin-dependent kinase inhibitor, p27 Kip1 is a tumor suppressor protein that acts in the nucleus to enforce cell cycle checkpoints. Like its relatives, p57 Kip2 and p21 Waf1/Cip1, the ability to enforce the G1 restriction point is derived from its inhibitory binding to CDK2/cyclin E and other CDK/cyclin complexes. Expression levels of p27 are upregulated in quiescent cells and in cells treated with cAMP or other negative cell cycle regulators. Downregulation of p27 can be induced by treatment with interleukin-2 or other mitogens; this involves phosphorylation of p27 and its degradation by the ubiquitin-proteasome pathway (1-4).
  • Bibliography
    1. Lloyd, R.V. et al. (1999) Am J Pathol 154, 313-23. 2. Polyak, K. et al. (1994) Genes Dev 8, 9-22. 3. Kato, J.Y. et al. (1994) Cell 79, 487-96. 4. Vlach, J. et al. (1997) EMBO J 16, 5334-44.
  • Released date
    6/Sep/2016
  • NCBI number
    NP_004055.1
  • Gene number
    1027
  • Protein number
    P46527
  • PubMed number
    Refer to PubMed
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • Antibody s datasheet
    Contact our support service to receive datasheet or other technical documentation.
  • Representative figure link
    Representative_figure_link
  • Representative figure legend
    Immunohistochemistry analysis using Rabbit Anti-p27 Kip1 Polyclonal Antibody (SPC-1282). Tissue: Brain tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-p27 Kip1 Polyclonal Antibody (SPC-1282) at 1:100. The image on the right is treated with the synthesized peptide. Rabbit Anti-p27 Kip1 Antibody used in Immunohistochemistry (IHC) on Brain tissue (SPC-1282)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availabilit
    In Stock
  • Other related products
    Other_related_products
  • Gene target
    p27 Kip1 ATTO 633
  • Host
    Rabbit, Rabbits
  • isotype filter
    • IgG
  • Short name
    Rabbit Anti- p27 Kip1 Polyclonal: ATTO 633
  • label filter
    • ATTO 633
  • technique filter
    • anti-Human
    • Polyclonal
    • Rabbit
    • anti-
    • anti
  • Technique
    anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Alternative technique
    polyclonals, rabbit-anti, antibodies