Rabbit Anti-Human MMP 2 Polyclonal: ATTO 488

• Catalog number
  SPC-1278D-A488
• Price
  Please ask
• Size
  100 µl

• Immunogen
  Synthesized peptide derived from human MMP-2.
• Antibody s target
  Human MMP 2
• Antibody s full description
  Rabbit Anti-Human MMP 2 Polyclonal
• Primary research fields
  Cardiovascular System, Angiogenesis, Growth Factors, Adhesion / Extracellular Matrix, Matrix Metalloproteinases, Cancer, Invasion/microenvironment, Cell Signaling, Metabolism, Metabolism processes, Hypoxia
• Antibody s category
  Polyclonal Antibodies
• Antibody s other name
  72 kDa gelatinase Antibody, CLG 4 Antibody, Collagenase Type 4 alpha Antibody, Matrix metallopeptidase 2 gelatinase A 72kDa Antibody, Matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase) Antibody, Matrix Metalloproteinase 2 Antibody, MMP II Antibody, MONA Antibody, PEX Antibody, TBE 1 Antibody
• Verified applications
  WB, ELISA
• Raised in
  Rabbit
• Antibody s reactivity
  Human, Mouse, Rat
• Antibody s recommended dilutions for use
  WB (1:1000), ELISA (1:20000); optimal dilutions for assays should be determined by the user.
• Antibody s purified from
  Peptide Affinity Purified
• Recommended buffer for storage
  PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
• Antibody s concentration
  See included datasheet or contact our support service
• Antibody s specificity
  Detects ~72kDa, endogenous levels of total MMP-2 protein.
• Storage recommendations
  -20°C
• Shipping recommendations
  Blue Ice or 4°C
• Certificate of analysis
  A 1:1000 dilution of SPC-1278 was sufficient for detection of MMP 2 in 10 µg of LOVO cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
• Antibody in cell
  Extracellular matrix, Membrane, Nucleus
• Tissue specificity
  Produced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate.
• Scientific context
  Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the ability to degrade all the components of the extracellular matrix. Specifically, MMP2, the signaling mechanism for cells to begin migration, triggers the movement when it splits laminin-5 and exposes and integrin-binding site on this component of epithelial basement membrane. The changed form of laminin was found in tumors and in tissues being altered. The activated form of MMP2, however, is not found in benign tumors. Thus detection of the enzyme is a possible early indicator of tumor activity.
• Bibliography
  1. Cawston T.E. (2004) in Interstitial Collagenase. Barrett, A.J. et al. (eds): Handbook of Proteolytic Enzymes, San Diego: Academic Press, p. 472. 2. Coussens L.M., et al. (2002) Science. 295: 2387-92. 3. Sternlicht M.D., et al. (1999) Cell. 98: 137-46. 4. Vu T.H., et al. (1998) Cell. 93: 411-22.
• Released date
  6/Sep/2016
• NCBI number
  NP_004521.1
• Gene number
  4313
• Protein number
  P08253
• PubMed number
  Refer to PubMed
• Tested applications
  To be tested
• Tested reactivity
  To be tested
• Antibody s datasheet
  Contact our support service to receive datasheet or other technical documentation.
• Representative figure link
• Representative figure legend
  Western blot analysis of Human LOVO cell lysates showing detection of ~72kDa MMP 2 protein using Rabbit Anti-MMP 2 Polyclonal Antibody (SPC-1278). Lane 1: Human LOVO cells. Lane 2: Human LOVO cells treated with the immunizing peptide. Primary Antibody: Rabbit Anti-MMP 2 Polyclonal Antibody (SPC-1278) at 1:1000. Predicted/Observed Size: ~72kDa. Rabbit Anti-MMP 2 Antibody used in Western blot (WB) on LOVO cell lysates (SPC-1278)
• Warning information
  Non-hazardous
• Country of production
  Canada
• Total weight kg
  1.4
• Net weight g
  0.1
• Stock availabilit
  In Stock
• Other related products
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Test
  StressMark antibodies supplies antibodies that are for research of human proteins.
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
• Latin name
  Oryctolagus cuniculus

• Gene target
  MMP   ATTO   488  
• Gene symbol
  MMP24, MMP14, MMP28, MMP17, MMP15, MMP25, MMP16
• Short name
  Rabbit Anti- MMP 2 Polyclonal: ATTO 488
• Technique
  anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Host
  Rabbit, Rabbits
• Isotype
  IgG
• Label
  ATTO 488
• Species
  Human, Humans
• Alternative name
  production species: rabbit Antibody toHuman MMP 2 polyclonal: ATTO 488
• Alternative technique
  polyclonals, rabbit-anti, antibodies

Gene info

• Identity
  HGNC:7172
• Gene
  MMP24
• Long gene name
  matrix metallopeptidase 24
• Synonyms gene name
  • matrix metalloproteinase 24 (membrane-inserted)
  • matrix metallopeptidase 24 (membrane-inserted)
• Synonyms
  • MT5-MMP
• Synonyms name
  • membrane-type 5 matrix metalloproteinase
• GenBank acession
  • AF131284
• Locus
  20q11.22
• Discovery year
  1999-08-06
• Entrez gene record
  10893
• Pubmed identfication
  • 10363975
• RefSeq identity
  • NM_006690
• Classification
  • M10 matrix metallopeptidases
• VEGA ID
  OTTHUMG00000032330

Gene info

• Identity
  HGNC:7160
• Gene
  MMP14
• Long gene name
  matrix metallopeptidase 14
• Synonyms gene name
  • matrix metalloproteinase 14 (membrane-inserted)
  • matrix metallopeptidase 14 (membrane-inserted)
• Synonyms
  • MT1-MMP
• Synonyms name
  • membrane type 1 metalloprotease
  • membrane type 1-matrix metalloproteinase
• Locus
  14q11.2
• Discovery year
  1994-11-20
• Entrez gene record
  4323
• Pubmed identfication
  • 8015608
• RefSeq identity
  • NM_004995
• Classification
  • M10 matrix metallopeptidases
• VEGA ID
  OTTHUMG00000028704

Gene info

• Identity
  HGNC:14366
• Gene
  MMP28
• Long gene name
  matrix metallopeptidase 28
• Synonyms gene name
  • matrix metalloproteinase 28
• Synonyms
  • MMP-25
  • MM28
  • EPILYSIN
  • MMP-28
• GenBank acession
  • AF315683
• Locus
  17q12
• Discovery year
  2001-01-09
• Entrez gene record
  79148
• Pubmed identfication
  • 11121398
  • 11255011
• RefSeq identity
  • NM_024302
• Classification
  • M10 matrix metallopeptidases
• VEGA ID
  OTTHUMG00000188387

Gene info

• Identity
  HGNC:7163
• Gene
  MMP17
• Long gene name
  matrix metallopeptidase 17
• Synonyms gene name
  • matrix metalloproteinase 17 (membrane-inserted)
  • matrix metallopeptidase 17 (membrane-inserted)
• Synonyms
  • MT4-MMP
• GenBank acession
  • X89576
• Locus
  12q24.33
• Discovery year
  1996-11-13
• Entrez gene record
  4326
• Pubmed identfication
  • 9878265
• RefSeq identity
  • NM_016155
• Classification
  • M10 matrix metallopeptidases
• VEGA ID
  OTTHUMG00000168050

Gene info

• Identity
  HGNC:7161
• Gene
  MMP15
• Long gene name
  matrix metallopeptidase 15
• Synonyms gene name
  • matrix metalloproteinase 15 (membrane-inserted)
  • matrix metallopeptidase 15 (membrane-inserted)
• Synonyms
  • MT2-MMP
  • MTMMP2
  • SMCP-2
• GenBank acession
  • Z48482
• Locus
  16q21
• Discovery year
  1996-11-13
• Entrez gene record
  4324
• Pubmed identfication
  • 9070935
  • 9119382
• RefSeq identity
  • NM_002428
• Classification
  • M10 matrix metallopeptidases
• VEGA ID
  OTTHUMG00000133466

Gene info

• Identity
  HGNC:14246
• Gene
  MMP25
• Long gene name
  matrix metallopeptidase 25
• Synonyms gene
  • MMPL1
  • MMP20
• Synonyms gene name
  • matrix metalloproteinase 25
  • matrix metallopeptidase-like 1
• Synonyms
  • MT6-MMP
• GenBank acession
  • AF145442
• Locus
  16p13.3
• Discovery year
  2000-12-13
• Entrez gene record
  64386
• Pubmed identfication
  • 10628838
  • 10706098
• RefSeq identity
  • NM_022468
• Classification
  • M10 matrix metallopeptidases
• VEGA ID
  OTTHUMG00000177475

Gene info

• Identity
  HGNC:7162
• Gene
  MMP16
• Long gene name
  matrix metallopeptidase 16
• Synonyms gene
  • C8orf57
• Synonyms gene name
  • matrix metalloproteinase 16 (membrane-inserted)
  • chromosome 8 open reading frame 57
  • matrix metallopeptidase 16 (membrane-inserted)
• Synonyms
  • MT3-MMP
  • DKFZp761D112
• GenBank acession
  • D85511
• Locus
  8q21.3
• Discovery year
  1996-11-13
• Entrez gene record
  4325
• Pubmed identfication
  • 7559440
• RefSeq identity
  • NM_005941
• Classification
  • M10 matrix metallopeptidases
• VEGA ID
  OTTHUMG00000163769

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

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