Rabbit Anti-Human MMP 1 Polyclonal: ATTO 633
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Catalog numberSPC-1277D-A633
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PricePlease ask
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Size100 µl
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ImmunogenA synthesized peptide derived from human MMP1
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Antibody s targetHuman MMP 1
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Antibody s full descriptionRabbit Anti-Human MMP 1 Polyclonal
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Primary research fieldsCardiovascular System, Angiogenesis, Growth Factors, Adhesion / Extracellular Matrix, Matrix Metalloproteinases, Cell Signaling, Extracellular Matrix (ECM), Enzymes, Cancer, Invasion/microenvironment
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Antibody s categoryPolyclonal Antibodies
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Antibody s other name27 kDa interstitial collagenase Antibody, CLG Antibody, CLGN Antibody, Matrix metallopeptidase 1 (interstitial collagenase) Antibody, Matrix metalloprotease 1 Antibody, MMP 1 Antibody
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Verified applicationsWB
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Raised inRabbit
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Antibody s reactivityHuman
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Antibody s recommended dilutions for useWB (1:3000); optimal dilutions for assays should be determined by the user.
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Antibody s purified fromPeptide Affinity Purified
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Recommended buffer for storagePBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
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Antibody s concentrationSee included datasheet or contact our support service
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Antibody s specificityDetects ~54kDa, endogenous levels of total MMP1
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Storage recommendations-20°C
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Shipping recommendationsBlue Ice or 4°C
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Certificate of analysisA 1:1000 dilution of SPC-1277 was sufficient for detection of MMP 1 in 10 µg of HepG2 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
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Antibody in cellExtracellular matrix
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Tissue specificityExpressed in fibroblasts, keratinocytes, endothelial cells, monocytes, macrophages
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Scientific contextMatrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the ability to degrade all the components of the extracellular matrix. MMP1 in particular cleaves collagen types I, II, III and IV, as well as casein, gelatin, antitrypsin, myelin basic protein, L-Selectin, pro-TNF, IL-1 beta, IGF-BP3, IGF-BP5, pro MMP-2 and pro MMP-9. MMP1 is downregulated by p53, and abnormality of p53 expression may lead to joint degradation in rheumatoid arthritis.
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Bibliography1. Cawston T.E. (2004) in Interstitial Collagenase. Barrett, A.J. et al. (eds): Handbook of Proteolytic Enzymes, San Diego: Academic Press, p. 472.
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Released date6/Sep/2016
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NCBI numberNP_002412.1
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Gene number4312
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Protein numberP03956
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PubMed numberRefer to PubMed
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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Antibody s datasheetContact our support service to receive datasheet or other technical documentation.
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Representative figure legendWestern blot analysis of Human HepG2 cell lysates showing detection of ~54kDa MMP 1 protein using Rabbit Anti-MMP 1 Polyclonal Antibody (SPC-1277). Lane 1: Human HepG2 cell lysate treated with the immunizing peptide. Lane 2: Human HepG2 cell lysate. Primary Antibody: Rabbit Anti-MMP 1 Polyclonal Antibody (SPC-1277) at 1:1000. Predicted/Observed Size: ~54kDa. Rabbit Anti-MMP 1 Antibody used in Western blot (WB) on HepG2 cell lysates (SPC-1277)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilitIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestStressMark antibodies supplies antibodies that are for research of human proteins.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin nameOryctolagus cuniculus
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Gene target
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Gene symbolMMP24, MMP14, MMP28, MMP17, MMP15, MMP25, MMP16
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Short nameRabbit Anti- MMP 1 Polyclonal: ATTO 633
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Techniqueanti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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HostRabbit, Rabbits
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IsotypeIgG
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LabelATTO 633
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SpeciesHuman, Humans
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Alternative nameproduction species: rabbit Antibody toHuman MMP 1 polyclonal: ATTO 633
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Alternative techniquepolyclonals, rabbit-anti, antibodies
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Gene info
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Identity
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Gene
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Long gene namematrix metallopeptidase 24
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Synonyms gene name
- matrix metalloproteinase 24 (membrane-inserted)
- matrix metallopeptidase 24 (membrane-inserted)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1999-08-06
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- M10 matrix metallopeptidases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namematrix metallopeptidase 14
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Synonyms gene name
- matrix metalloproteinase 14 (membrane-inserted)
- matrix metallopeptidase 14 (membrane-inserted)
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Synonyms
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Synonyms name
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Locus
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Discovery year1994-11-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- M10 matrix metallopeptidases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namematrix metallopeptidase 28
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Synonyms gene name
- matrix metalloproteinase 28
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-01-09
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- M10 matrix metallopeptidases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namematrix metallopeptidase 17
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Synonyms gene name
- matrix metalloproteinase 17 (membrane-inserted)
- matrix metallopeptidase 17 (membrane-inserted)
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Synonyms
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GenBank acession
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Locus
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Discovery year1996-11-13
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- M10 matrix metallopeptidases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namematrix metallopeptidase 15
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Synonyms gene name
- matrix metalloproteinase 15 (membrane-inserted)
- matrix metallopeptidase 15 (membrane-inserted)
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Synonyms
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GenBank acession
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Locus
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Discovery year1996-11-13
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- M10 matrix metallopeptidases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namematrix metallopeptidase 25
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Synonyms gene
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Synonyms gene name
- matrix metalloproteinase 25
- matrix metallopeptidase-like 1
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-12-13
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- M10 matrix metallopeptidases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namematrix metallopeptidase 16
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Synonyms gene
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Synonyms gene name
- matrix metalloproteinase 16 (membrane-inserted)
- chromosome 8 open reading frame 57
- matrix metallopeptidase 16 (membrane-inserted)
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Synonyms
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GenBank acession
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Locus
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Discovery year1996-11-13
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- M10 matrix metallopeptidases
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data