Rabbit Anti-Human Met (pThr1355+pTyr1356) Polyclonal: ATTO 565
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Catalog numberSPC-1022D-A565
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Price:
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Size100 µl
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ImmunogenA phospho-specific peptide corresponding to residues surrounding Thr1355 and Tyr1356 of human Met (AA1353-1359)
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Antibody s targetHuman Met (pThr1355+pTyr1356)
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Antibody s full descriptionRabbit Anti-Human Met (pThr1355+pTyr1356) Polyclonal
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Antibody s categoryPolyclonal Antibodies
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Antibody s other nameAUTS9 antibody, c met antibody, D249 antibody, Hepatocyte growth factor receptor antibody, HGF antibody, HGFR antibody, MET antibody, Met proto oncogene tyrosine kinase antibody, MET_HUMAN antibody, Oncogene MET antibody, Par4 antibody, Proto-oncogene c-Met antibody, RCCP2 antibody, Scatter factor receptor antibody, SF receptor antibody, Tyrosine-protein kinase Met antibody
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Verified applicationsWB, AM
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Raised inRabbit
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Antibody s reactivityHuman
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Antibody s recommended dilutions for useWB (1:250); optimal dilutions for assays should be determined by the user.
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Antibody s purified fromPeptide Affinity Purified
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Recommended buffer for storagePBS pH7.4, 50% glycerol, 0.025% Thimerosal
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Antibody s concentrationSee included datasheet or contact our support service
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Antibody s specificityDetects 155.527 kDa.
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Storage recommendations-20°C
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Shipping recommendationsBlue Ice or 4°C
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Certificate of analysisA 1:250 dilution of SPC-1022 was sufficient for detection of Met (pThr1355+pTyr1356) in 10 µg of 293T cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cellMembrane
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Tissue specificityExpressed in normal hepatocytes as well as in epithelial cells lining the stomach, the small and the large intestine. Found also in basal keratinocytes of esophagus and skin. High levels are found in liver, gastrointestinal tract, thyroid and kidney. Also present in the brain.
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Scientific contextMET is a receptor-tyrosine kinase that is activated upon binding hepatocyte growth factor (HGF). MET/HGF signalling has been implicated in the generation and progression of a variety of tumours. The coexpression of wild type MET and HGF in the same cell (which generates an autocrine stimulatory loop) induces oncogenic transformation. All investigated naturally occurring MET mutants show increased tyrosine phosphorylation level and enhanced kinase activity
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BibliographyRefer to PubMed
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Released date29/Aug/2016
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NCBI numberNP_000236
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Gene number4233
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Protein numberP08581
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PubMed numberRefer to PubMed
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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Antibody s datasheetContact our support service to receive datasheet or other technical documentation.
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Representative figure linkNo Data Available
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilitIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestStressMark antibodies supplies antibodies that are for research of human proteins.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin nameOryctolagus cuniculus
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Gene target
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Short nameRabbit Anti- Met (pThr1355+pTyr1356) Polyclonal: ATTO 565
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Techniqueanti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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HostRabbit, Rabbits
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IsotypeImmunoglobulin
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LabelATTO 565
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SpeciesHuman, Humans
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Alternative nameproduction species: rabbit Antibody toHuman Met (pThr1355andpTyr1356) polyclonal: ATTO 565
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Alternative techniquepolyclonals, rabbit-anti, antibodies
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data