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Rabbit Anti-Human ULK1 Polyclonal: ATTO 565

  • Catalog number
    SPC-640D-A565
  • Product name
    Rabbit Anti-Human ULK1 Polyclonal: ATTO 565
  • Size
    100 µg
  • Label
    ATTO 565
  • Immunogen
    Synthetic peptide from the mid-protein of Human ULK1 (aa. 567-577)
  • Antibody s target
    Human ULK1
  • Antibody s full description
    Rabbit Anti-Human ULK1 Polyclonal
  • Primary research fields
    Neuroscience, Cell Signaling, Cardiovascular System, Heart, Cell Signaling, Protein Phosphorylation, Serine / Threonine Kinases, Cell Signaling, Metabolism, Mitochondrial Metabolism
  • Antibody s category
    Polyclonal Antibodies
  • Antibody s other name
    Unc-51 like kinase 1 (C. elegans) Antibody, Autophagy related protein 1 homolog Antibody, Autophagy-related protein 1 homolog Antibody, FLJ38455 Antibody, Serine/threonine-protein kinase ULK1 Antibody, Unc51.1 Antibody, ATG1A Antibody, UNC51, C. elegans, homolog of Antibody, EC:2.7.11.1 Antibody, Serine/threonine protein kinase Unc51.1 Antibody, FLJ46475 Antibody, ATG 1 Antibody, hATG1 Antibody, ATG1 Antibody, Unc-51-like kinase 1 Antibody, Serine/threonine protein kinase ULK1 Antibody, ULK1 Antibody, Unc 51 like kinase 1 Antibody, KIAA0722 Antibody, UNC 51 Antibody, ULK 1 Antibody, ULK1_HUMAN Antibody, UNC51 Antibody, Unc 51 (C. elegans) like kinase 1 Antibody, ATG1 autophagy related 1 homolog Antibody,
  • Verified applications
    WB, ICC/IF
  • Raised in
    Rabbit
  • Isotype
    Immunoglobulin
  • Antibody s reactivity
    Human, Mouse, Rat
  • Antibody s recommended dilutions for use
    WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
  • Antibody s purified from
    Peptide Affinity Purified
  • Recommended buffer for storage
    PBS, 50% glycerol, 0.09% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects ~100 kDa.
  • Storage recommendations
    -20°C
  • Shipping recommendations
    Blue Ice or 4°C
  • Certificate of analysis
    A 1:1000 dilution of SPC-640 was sufficient for detection of ULK1 in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
  • Antibody in cell
    Cytoplasm, Cytosol, Preautophagosomal Structure
  • Tissue specificity
    Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung.
  • Scientific context
    UNC-51 like kinase 1 (ULK1) is widely expresed and contains an amino-terminal kinase domain followed by a central proline-serine rich domain and a highly conserved carboxy-terminal domain. It has been linked to axon growth and is essential for autophagy. Structurally, ULK1 is similar to ATG1, and it appears that both Atg1/ULK1 can bind to several ATG proteins regulating phosphorylation states and protein trafficking.
  • Bibliography
    1. Okazaki N., et al. (2000) Brain Res Mol Brain Res. 85: 1-12. 2. Young A.R., et al. (2006) J Cell Sci. 119: 3888-900. 3. Kamada Y., et al. (2000) J Cell Biol. 150: 1507-13. 4. Lee S.B, et al. (2007) EMBO Rep. 8: 360-5. 5. Hara T., et al. (2008) J Cell Biol. 181: 497-510.
  • Released date
    29/Aug/2016
  • NCBI number
    NP_003556.1
  • Gene number
    8408
  • Protein number
    O75385
  • PubMed number
    Refer to PubMed
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • Antibody s datasheet
    Contact our support service to receive datasheet or other technical documentation.
  • Representative figure link
    Representative_figure_link
  • Representative figure legend
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ULK1 Polyclonal Antibody (SPC-640). Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ULK1 Polyclonal Antibody (SPC-640) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Preautophagosomal Structure. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ULK1 Antibody (D) Composite. | Western blot analysis of Human HeLa and 293Trap cell lysates showing detection of 112.6 kDa ULK1 protein using Rabbit Anti-ULK1 Polyclonal Antibody (SPC-640). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa and 293Trap cell lysates. Load: 15 µg . Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ULK1 Polyclonal Antibody (SPC-640) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 112.6 kDa. | Western blot analysis of Rat Brain cell lysates showing detection of ~112.6kDa ULK1 protein using Rabbit Anti-ULK1 Polyclonal Antibody (SPC-640). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 µg . Block: 2% GE Healthcare Blocker (RT, 60 minutes). Primary Antibody: Rabbit Anti-ULK1 Polyclonal Antibody (SPC-640) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1/2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~112.6kDa. Rabbit Anti-ULK1 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human SK-N-BE Cells (Human Neuroblastoma cells) (SPC-640) | Rabbit Anti-ULK1 Antibody used in Western blot (WB) on HeLa and 293Trap cell lysates (SPC-640) | Rabbit Anti-ULK1 Antibody used in Western blot (WB) on Brain cell lysates (SPC-640)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availabilit
    In Stock
  • Other related products
    Other_related_products
  • Gene target
    ULK1 ATTO 565
    • Gene info
  • Gene symbol
    ULK1
  • Host
    Rabbit, Rabbits
  • isotype filter
    • Immunoglobulin
  • Short name
    Rabbit Anti- ULK1 Polyclonal: ATTO 565
  • label filter
    • ATTO 565
  • technique filter
    • anti-Human
    • Polyclonal
    • Rabbit
    • anti-
    • anti
  • Technique
    anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Alternative technique
    polyclonals, rabbit-anti, antibodies