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Immunogen
C-terminal 238 amino acids of HSP90C expressed with N- and C-terminal hexahistidine tags in E.coli, purified with Ni-NTA.
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Antibody s target
Algae HSP90
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Antibody s full description
Rabbit Anti-Algae HSP90C (Plant) Polyclonal
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Primary research fields
Cancer, Heat Shock
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
HSP84 Antibody, HSP86 Antibody, HSP90A Antibody, HSP90AA1 Antibody, HSP90AB1 Antibody, HSP90B Antibody, HSPC1 Antibody, HSPC2 Antibody, HSPCAL1 Antibody, HSPCAL4 Antibody, HSP90N Antibody
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Verified applications
WB, IP
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Raised in
Rabbit
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Antibody s reactivity
Algae, Algae (Chlamydomonas reinhardtii)
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Antibody s recommended dilutions for use
WB (1:3000); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Rabbit Antiserum
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Recommended buffer for storage
Lyophilized rabbit Antiserum. For reconstitution add 100 µl of sterile water.
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects ~70kDa. HSP90C is easily degraded and the antibody detects these degradation fragments.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
Contact our support service for certificate of analysis
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Antibody in cell
Cytoplasm, Melanosome
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Tissue specificity
See included datasheet or contact our support service.
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Scientific context
HSP90C is a nuclear-encoded, chloroplast-targeted chaperone of the HSP90 family. In the chloroplast, it is localized to the soluble stroma fraction, but also to thylakoids and low-density membranes containing inner envelopes. HSP90C is expressed under basal conditions and is strongly induced by heat shock and moderately by light. It forms dimers that interact with HSP70B. HSP90C displays a weak ATPase activity that is inhibited by radicicol (1). Looking for more information on HSP90? Visit our new HSP90 Scientific Resource Guide at more.
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Bibliography
1. Willmund F., and Schroda M. (2005) Plant Phys. 138: 2310-2322.
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Released date
1/Jan/2009
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NCBI number
XP_001702984.1
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Gene number
5728553
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Protein number
Q66T67
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
No Data Available
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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About
Plants RNA, DNA , cDNA and plant tissues are used to study plant genes. Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Group
Polyclonals and antibodies
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Latin name
Oryctolagus cuniculus
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Conjugation
not conjugated
