Rabbit Anti-Algae HSP70B (Plant) Polyclonal: Unconjugated

• Catalog number
  SPC-315D
• Price
  Please ask
• Size
  100 µl

• Immunogen
  Mature HSP70B protein expressed with N- and C- terminal hexahistidine tags in E.coli, purified with Ni-NTA
• Antibody s target
  Algae HSP70
• Antibody s full description
  Rabbit Anti-Algae HSP70B (Plant) Polyclonal
• Primary research fields
  Cancer, Heat Shock
• Antibody s category
  Polyclonal Antibodies
• Antibody s other name
  HSP70 1 Antibody, HSP70 2 Antibody, HSP70.1 Antibody, HSP72 Antibody, HSPA1 Antibody, HSPA1A Antibody, HSPA1B Antibody
• Verified applications
  WB, IP
• Raised in
  Rabbit
• Antibody s reactivity
  Algae, Plant, Algae (Chlamydomonas reinhardtii), Algae (Desmodesmus subspicatus), Moss (Physcomitrella patens)
• Antibody s recommended dilutions for use
  WB (1:10000); optimal dilutions for assays should be determined by the user.
• Antibody s purified from
  Rabbit Antiserum
• Recommended buffer for storage
  Lyophilized rabbit Antiserum. For reconstitution add 100 µl of sterile water.
• Antibody s concentration
  See included datasheet or contact our support service
• Antibody s specificity
  Detects ~70kDa.
• Storage recommendations
  -20°C
• Shipping recommendations
  Blue Ice or 4°C
• Certificate of analysis
  Contact our support service for certificate of analysis
• Antibody in cell
  Cytoplasm
• Tissue specificity
  See included datasheet or contact our support service.
• Scientific context
  HSP70B (heat shock protein 70B) is a nuclear-encoded, chloroplast-targeted chaperone of the HSP70 family. It is the major HSP70 in the stroma of Chlamydomonas chloroplasts. It interacts with HSP90C, CGE1, CDJ2 and VIPP1 (1-3). It has been shown that using Chlamydomonas reinhardtii as plant model organism the alga encodes a HEP homolog (termed HEP2) that is localized to the stroma (4). HEP2 is expressed constitutively as a low abundance protein with an apparent molecular mass of ~21 kDa. In cell extracts HEP2 interacts with HSP70B in an ATP-dependent fashion. Coexpression of HSP70B with HEP2 in E. coli yields high levels of CGE1- binding competent HSP70B, which also displayed ATPase activity (4). Inactive HSP70B was more prone to proteolysis than active HSP70B. Although inactive HSP70B interacts with HEP2, it is not activated. Active HSP70B remains active for 48 h in the absence of HEP2, suggesting that HEP2 was not involved in maintaining HSP70B in an active state. However, it was found that some HSP70B expressed as a fusion protein with an N-terminal extension was activated when HEP2 was present during cleavage of the fusion protein, suggesting that in vivo HEP2 might be required for renewed folding of HSP70B after transit peptide cleavage (4). Looking for more information on HSP70? Visit our new HSP70 Scientific Resource Guide at more.
• Bibliography
  1. Schroda M., Vallon O., Wollman F.A. and Beck C.F. (1999) Plant Cell. 11: 1165-1178. 2. Schroda M., et al. (2001) Plant Cell. 13: 2823-2839. 3. Liu C., et al. (2005) Mol Biol Cell. 16: 1165-1177. 4. Willund F., et al. (2008) J. Biol. Chem., 283(24): 16363-16373.
• Released date
  1/Jan/2009
• NCBI number
  XP_001696432.1
• Gene number
  5722220
• Protein number
  A8HYV3
• PubMed number
  Refer to PubMed
• Tested applications
  To be tested
• Tested reactivity
  To be tested
• Antibody s datasheet
  Contact our support service to receive datasheet or other technical documentation.
• Representative figure link
  No Data Available
• Warning information
  Non-hazardous
• Country of production
  Canada
• Total weight kg
  1.4
• Net weight g
  0.1
• Stock availabilit
  In Stock
• Other related products
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• About
  Plants RNA, DNA , cDNA and plant tissues are used to study plant genes. Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
• Group
  Polyclonals and antibodies
• Latin name
  Oryctolagus cuniculus
• Conjugation
  not conjugated 

• Gene target
  Algae   HSP70B   Unconjugated  
• Gene symbol
  HSPA6, HSPA7
• Short name
  Rabbit Anti-Algae HSP70B ( ) Polyclonal: Unconjugated
• Technique
  Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Host
  Rabbit, Rabbits
• Isotype
  Immunoglobulin
• Label
  Unconjugated
• Species
  Plant, Plants
• Alternative name
  production species: rabbit Antibody toAlgae HSP70B (vegetal) polyclonal: nonconjugated
• Alternative technique
  polyclonals, rabbit-anti, antibodies

Gene info

• Identity
  HGNC:5239
• Gene
  HSPA6
• Long gene name
  heat shock protein family A (Hsp70) member 6
• Synonyms gene name
  • heat shock 70kD protein 6 (HSP70B')
  • heat shock 70kDa protein 6 (HSP70B')
• Synonyms
  • HSP70B'
• Locus
  1q23.3
• Discovery year
  1991-07-26
• Entrez gene record
  3310
• Pubmed identfication
  • 1346391
• RefSeq identity
  • NM_002155
• Classification
  • Heat shock 70kDa proteins
• VEGA ID
  OTTHUMG00000034461

Gene info

• Identity
  HGNC:5240
• Gene
  HSPA7
• Long gene name
  heat shock protein family A (Hsp70) member 7 (pseudogene)
• Synonyms gene name
  • heat shock 70kD protein 7 (HSP70B)
  • heat shock 70kDa protein 7 (HSP70B)
  • heat shock protein family A (Hsp70) member 7
• Synonyms
  • HSP70B
• Locus
  1q23.3
• Discovery year
  1991-07-26
• Entrez gene record
  3311
• Pubmed identfication
  • 1346391
  • 11072087
• RefSeq identity
  • NR_024151
• Classification
  • Heat shock 70kDa proteins
• VEGA ID
  OTTHUMG00000074098

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

Similar products