Mouse Anti-Rat VGLUT2 Monoclonal IgG1, Clone S29-29
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Catalog numberSMC-395D-A565
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PricePlease ask
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Size100 µg
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CloneS29-29
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ImmunogenFusion protein amino acids 501-582 (cytoplasmic C-terminus) of rat VGlut2
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Antibody s full descriptionMouse Anti-Rat VGLUT2 Monoclonal IgG1 Antibody, Clone: S29-29: ATTO 565
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameDifferentiation associated BNPI Antibody, DNPI Antibody, SLC17A6 Antibody, Solute carrier family 17 member 6 Antibody, Vesicular glutamate transporter 2 Antibody, Differentiation associated Na dependent inorganic phosphate cotransporter Antibody, Solute carrier family 17 (Sodium dependent inorganic phosphate cotransporter) member 6 Antibody, Differentiation associated Na(+) dependent inorganic phosphate cotransporter Antibody, Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter Antibody, Sodium dependent inorganic phosphate cotransporter Antibody
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Raised inMouse
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Antibody s targetVGLUT2
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Primary research fieldsNeuroscience, Cell Structure, Pre-Synaptic Markers, Pumps/Transporters, Neurotransmitter Transporters, Cell Signaling
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BrandnameNone
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Antibodies applicationsWB, IHC, ICC/IF
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Antibody s reactivityHuman, Mouse, Rat
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Antibody s dilutionsWB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~60kDa.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysis1 µg/ml of SMC-395 was sufficient for detection of VGLut2 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellCytoplasmic Vesicle , Secretory Vesicle , Synaptic Vesicle Membrane , Membrane , Cell Junction , Synapse
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextThe ATP-dependent, chloride-sensitive vesicular glutamate transporters (VGLUT) include BNPI (VGLUT1), VGLUT2 (DNPI) and VGLUT3. The brain expresses BNPI (brain specific Na+-dependent inorganic phosphate (Pi) cotransporter) and VGLUT2 in a complementary fashion. The telencephalic regions express BNPI, whereas the lower brainstem and diencephalic regions express VLGUT2. Rat pinealocytes express both BNPI and VGLUT2. The striatum, hippocampus, cerebral cortex and raphe nuclei express VGLUT3 in a small number of neurons. Pancreatic α and β cells express BNPI and VGLUT2 in response to glucose concentrations. Human VGLUT3 shares a 72% sequence homology with VLGUT2 and BNPI.
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Bibliography1. Aihara Y., et al. (2000) J Neurochem. 74: 2622-2625. 2. Kaneko T., and Fujiyama F. (2002) Neurosci Res. 42: 243-250. 3. Gras C., et al. (2002) J Neurosci. 22: 5442-5451. 4. Takamori S., Malherbe P., Broger C., and Jahn R. (2002) EMBO Rep. 3: 798-803. 5. Morimoto R., Hayashi M., Yatsushiro S., Otsuka M., Yamamoto A., and Moriyama Y. (2003) J Neurochem. 84: 382-391. 6. Bai L., Zhang X., and Ghishan F.K. (2003) Am. J. Physiol. Gastrointest. Liver Physiol. 284: G808-814.
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Released date16-May-2012
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_445879.1
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Gene number84487
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Protein numberQ9JI12
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-VGLUT2 Monoclonal Antibody, Clone S29-29 (SMC-395). Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-VGLUT2 Monoclonal Antibody (SMC-395) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasmic Vesicle, Secretory Vesicle, Synaptic Vesicle Membrane, Membrane, Cell Junction, Synapse. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) VGLUT2 Antibody (D) Composite. Western Blot analysis of Rat brain membrane lysate showing detection of VGLUT2 protein using Mouse Anti-VGLUT2 Monoclonal Antibody, Clone S29-29 (SMC-395). Primary Antibody: Mouse Anti-VGLUT2 Monoclonal Antibody (SMC-395) at 1:000. Mouse Anti-VGLUT2 Antibody [S29-29] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human SK-N-BE Cells (Human Neuroblastoma cells) (SMC-395) Mouse Anti-VGLUT2 Antibody [S29-29] used in Western Blot (WB) on Rat brain membrane lysate (SMC-395)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus, Rattus norvegicus
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Gene target
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Gene symbolSNORD114-29, SNORD115-29, SNORD116-29, RPS29, SLC17A6, IGLV3-29, IGHV3-29, IGKV2-29, IGKV2D-29, ERVK-29
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Short nameMouse Anti- VGLUT2 Monoclonal IgG1, Clone S29-29
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Techniqueanti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG1, IgG1
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LabelATTO 565
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SpeciesRat, Mouses, Rats
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Alternative nameMouse Antibody toRat VGLUT2 monoclonal IgG1, clonality S29-29
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Alternative techniquemurine, antibodies
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Clone nameS29-29
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Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 114-29
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Synonyms
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Locus
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Discovery year2006-09-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 115-29
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 116-29
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameribosomal protein S29
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Synonyms
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GenBank acession
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Locus
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Discovery year1997-07-04
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- S ribosomal proteins
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namesolute carrier family 17 member 6
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Synonyms gene name
- solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6
- solute carrier family 17 (vesicular glutamate transporter), member 6
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2001-09-27
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Solute carriers
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin lambda variable 3-29 (pseudogene)
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Synonyms gene name
- immunoglobulin lambda variable 3-29
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GenBank acession
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Locus
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Discovery year2000-05-08
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin lambda locus at 22q11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 3-29 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable 3-29
- immunoglobulin heavy variable 3-29 pseudogene
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 2-29
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Synonyms gene name
- immunoglobulin kappa variable 2-29 (gene/pseudogene)
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 2D-29
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameendogenous retrovirus group K member 29
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Synonyms gene name
- endogenous retrovirus group K, member 29
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GenBank acession
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Locus
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Discovery year2014-01-03
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Pubmed identfication
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
