Mouse Anti-Rat TrpV3 Monoclonal IgG2a, Clone S15-39
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Catalog numberSMC-334D-A488
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PricePlease ask
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Size100 µg
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CloneS15-39
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ImmunogenSynthetic peptide amino acids 458-474 (cytoplasmic C-terminus) of rat TrpV3
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Antibody s full descriptionMouse Anti-Rat TRPV3 Monoclonal IgG2a Antibody, Clone: S15-39: ATTO 488
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameVRL3 Antibody, vanilloid receptor 2 Antibody, transient receptor potential cation channel subfamily V member 3 Antibody, sensitive channel TRPV3 Antibody, 1110036I10Rik Antibody, MGC124324 Antibody, MGC124325 Antibody, Trpv3 heat sensitive channel Antibody, vanilloid receptor-related osmotically activated channel protein Antibody
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Raised inMouse
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Antibody s targetTRPV3
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Primary research fieldsNeuroscience, Ion Channels, Transient Receptor Potential Channels
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BrandnameNone
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Antibodies applicationsWB, IHC, ICC/IF, IP, AM
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Antibody s reactivityHuman, Mouse, Rat
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Antibody s dilutionsWB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~70kDa.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysis1 µg/ml of SMC-334 was sufficient for detection of TrpV3 in 10 µg of COS-1 cell lysate transiently transfected with TrpV3 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody
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Antibody in cellMembrane
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextThe TRPV3 protein belongs to a family of non-selective cation channels that function in a variety of processes, including temperature sensation and vasoregulation. The thermo sensitive members of this family are expressed in subsets of sensory neurons that terminate in the skin, and are activated at distinct physiological temperatures. This channel is activated at temperatures between 22 and 40 degrees C. The gene lies in close proximity to another family member (TRPV1) gene on chromosome 17, and the two encoded proteins are thought to associate with each other to form heteromeric channels (1, 2).
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Bibliography1. Masamoto Y., Kawabata F., Fushiki T. (2009) Biosci. Biotechnol. Biochem. 73(5): 1021-1027. 2. Xiao R., et al. (2008) J Biol Chem. 283(10): 6162-6174.
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Released date29-Jun-2010
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_001020928
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Gene number497948
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Protein numberQ4QYD9
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunohistochemistry analysis using Mouse Anti-TrpV3 Monoclonal Antibody, Clone S15-39 (SMC-334). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-TrpV3 Monoclonal Antibody (SMC-334) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Filaggrin-like staining in upper layers. Dull lower layer cell staining. Some stain seen in hypodermis. Western Blot analysis of Rat brain membrane lysate showing detection of TrpV3 protein using Mouse Anti-TrpV3 Monoclonal Antibody, Clone S15-39 (SMC-334). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-TrpV3 Monoclonal Antibody (SMC-334) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TrpV3 Monoclonal Antibody, Clone S15-39 (SMC-334). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-TrpV3 Monoclonal Antibody (SMC-334) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Dotty staining in all cells. Some intermediate filament-like staining in some cells. Mouse Anti-TrpV3 Antibody [S15-39] used in Immunohistochemistry (IHC) on Mouse backskin (SMC-334) Mouse Anti-TrpV3 Antibody [S15-39] used in Western Blot (WB) on Rat brain membrane lysate (SMC-334) Mouse Anti-TrpV3 Antibody [S15-39] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HaCaT cells (SMC-334)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus, Rattus norvegicus
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Gene target
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Gene symbolSNORD115-39, IGKV1-39, IGHV4-39, IGKV1D-39, ZNF140, RPS15, VIPAS39, CHI3L2, RNU6-39P, IGLV5-39
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Short nameMouse Anti- TrpV3 Monoclonal IgG2a, Clone S15-39
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Techniqueanti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG2a, IgG2a
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LabelATTO 488
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SpeciesRat, Mouses, Rats
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Alternative nameMouse Antibody toRat TrpV3 monoclonal IgG2a, clonality S15-39
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Alternative techniquemurine, antibodies
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Clone nameS15-39
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Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 115-39
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 1-39
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Synonyms gene name
- immunoglobulin kappa variable 1-39 (gene/pseudogene)
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 4-39
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 1D-39
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namezinc finger protein 140
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Synonyms gene name
- zinc finger protein 140 (clone pHZ-39)
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Synonyms
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GenBank acession
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Locus
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Discovery year1993-02-11
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Zinc fingers C2H2-type
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameribosomal protein S15
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Synonyms
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Synonyms name
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Locus
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Discovery year1992-04-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- S ribosomal proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameVPS33B interacting protein, apical-basolateral polarity regulator, spe-39 homolog
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Synonyms gene
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Synonyms gene name
- chromosome 14 open reading frame 133
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2003-01-28
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namechitinase 3 like 2
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Synonyms gene name
- chitinase 3-like 2
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Synonyms
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GenBank acession
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Locus
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Discovery year1994-12-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Chitinases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameRNA, U6 small nuclear 39, pseudogene
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Synonyms gene
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Synonyms gene name
- RNA, U6 small nuclear 39
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Locus
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Discovery year2008-06-10
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Entrez gene record
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RefSeq identity
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin lambda variable 5-39
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GenBank acession
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Locus
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Discovery year2000-05-08
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Entrez gene record
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Classification
- Immunoglobulin lambda locus at 22q11.2
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data