Rat anti Mouse IgG2a Heavy Chain (biotin)

• Catalog number
  61R-I115aBT
• Price
  Please ask
• Size
  1 mg

• Applications
  ELISA
• Concentration
  1.0 mg/ml
• Product Subtype
  Conjugated Secondary Antibodies
• Research Area
  Immunology
• Product Type
  Secondary Antibodies
• Immunogen
  Mouse IgG2a heavy chain antibody (biotin) was raised in rat using murine IgG as the immunogen.
• Specificity
  Murine
• Form Buffer
  PBS with 0.1% NaN3 and 50% glycerol.
• Storage
  Aliquot and store at -20 deg C. Avoid repeated Freeze/Thaw cycles
• Shipping Info
  Blue Ice
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. This is against a protein of mus musculus directed antibody.
• Properties
  Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
• Conjugation
  Biotinylated
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus, Rattus norvegicus
• About
  Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

• Gene target
  IgG2a   Heavy   Chain  
• Short name
  anti Mouse IgG2a Heavy Chain ( )
• Technique
  anti mouse, Mouse, anti, antibody to, anti mouse antibodies, mouses
• Host
  Rat
• Isotype
  IgG1 kappa, IgG2a
• Label
  Biotin
• Species
  Mouse, Mouses, Rats
• Alternative name
  Rat anti Mouse IgG2a Heavy Chain antibody (biotin)
• Alternative technique
  murine, antibodies

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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