Mouse Anti-Rat SHANK1 Monoclonal IgG1, Clone S22-21
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Catalog numberSMC-329D-FITC
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PricePlease ask
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Size100 µg
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CloneS22-21
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ImmunogenFusion protein amino acids 469-691 (SH3/PDZ domains) of rat Shank1
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Antibody s full descriptionMouse Anti-Rat SHANK1 Monoclonal IgG1 Antibody, Clone: S22-21: FITC
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameGKAP/SAPAP interacting protein Antibody, OTTHUMP00000174437 Antibody, SH3 and multiple ankyrin repeat domains 1 Antibody, SH3 and multiple ankyrin repeat domains protein 1 Antibody, SH3/ankyrin domain gene 1 Antibody, SHAN1_HUMAN Antibody, SHANK 1 Antibody, Shank1 Antibody, Shank1a Antibody, Somatostatin receptor interacting protein Antibody, Somatostatin receptor-interacting protein Antibody, SPANK 1 Antibody, SPANK1 Antibody, SSTR interacting protein Antibody, SSTR-interacting protein Antibody, SSTRIP Antibody, Synamon Antibody
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Raised inMouse
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Antibody s targetSHANK1
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Primary research fieldsNeuroscience, Cell Structure, Post-Synaptic Markers, Cell Signaling, Scaffolds
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BrandnameNone
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Antibodies applicationsWB, IHC, AM
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Antibody s reactivityHuman, Mouse, Rat
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Antibody s dilutionsWB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~190-220 kDa (alternative splice variants). No cross-reactivity against Shank2 or Shank3.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysis1 µg/ml of SMC-329 was sufficient for detection of Shank1 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellCytoplasm , Cell Junction , Synapse , Postsynaptic Cell Membrane , Postsynaptic Density
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextShank proteins make up a family of scaffold proteins identified through their interaction with a variety of membrane and cytoplasmic proteins (1). Shank proteins at postsynaptic sites of excitatory synapses play roles in signal transmission into the postsynaptic neuron. Studies suggest that Shank2 is expressed in the neurons of the developing retina, and could play a role in the neuronal differentiation of the developing retina (2). Other recent studies suggest that the disruption of glutamate receptors at the Shank postsynaptic platform could contribute to the destruction of the postsynaptic density, which underlies the synaptic dysfunction and loss in Alzheimer’s disease (3).
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Bibliography1. Sheng M., and Kim E. (2000) Journal of Cell Science. 113: 1851-1856. 2. Kim J.H., et al. (2009) Exp Mol Med. 41(4): 236-242. 3. Gong Y., et al. (2009) Brain Res. 1292: 191-198.
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Released date13-Apr-2010
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_113939.2
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Gene number78957
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Protein numberQ9WV48
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunohistochemistry analysis using Mouse Anti-SHANK1 Monoclonal Antibody, Clone S22-21 (SMC-329). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-SHANK1 Monoclonal Antibody (SMC-329) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Filaggrin-like staining (upper layer aggregations of staining). Western Blot analysis of Rat brain membrane lysate showing detection of SHANK1 protein using Mouse Anti-SHANK1 Monoclonal Antibody, Clone S22-21 (SMC-329). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SHANK1 Monoclonal Antibody (SMC-329) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Mouse Anti-SHANK1 Antibody [S22-21] used in Immunohistochemistry (IHC) on Mouse backskin (SMC-329) Mouse Anti-SHANK1 Antibody [S22-21] used in Western Blot (WB) on Rat brain membrane lysate (SMC-329)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus, Rattus norvegicus
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Gene target
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Gene symbolPIRC105, SNORD115-21, HTOR, SNORD116-21, SNORD114-21, IGHV3-21
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Short nameMouse Anti- SHANK1 Monoclonal IgG1, Clone S22-21
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Techniqueanti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG1, IgG1
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LabelFITC
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SpeciesRat, Mouses, Rats
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Alternative nameMouse Antibody toRat SHANK1 monoclonal IgG1, clonality S22-21
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Alternative techniquemurine, antibodies
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Clone nameS22-21
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Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 105
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 115-21
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene name5-hydroxytryptamine (serotonin) oxygenase regulator
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 116-21
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 114-21
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Synonyms
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Locus
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Discovery year2006-09-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 3-21
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
