Mouse Anti-Rat Kir2.2 Monoclonal IgG1, Clone S124B-38

• Catalog number
  SMC-311D-PCP
• Price
  Please ask
• Size
  100 µg

• Clone
  S124B-38
• Immunogen
  Fusion protein amino acids 362-427 of rat Kir2.2
• Antibody s full description
  Mouse Anti-Rat Kir2.2 Monoclonal IgG1 Antibody, Clone: S124B-38: PerCP
• Antibody s category
  Monoclonal Antibodies
• Antibody s other name
  IRK2 Antibody, KCNJ12 Antibody, kcnj12x Antibody, KCNJN1 Antibody, Kir2.2v Antibody, potassium inwardly rectifying channel subfamily J member 12 Antibody
• Raised in
  Mouse
• Antibody s target
  Kir2.2
• Primary research fields
  Neuroscience, Ion Channels, Potassium Channels, Inward-Rectifying Potassium Channels
• Brandname
  None
• Antibodies applications
  WB, IHC, ICC/IF, AM
• Antibody s reactivity
  Human, Mouse, Rat
• Antibody s dilutions
  WB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
• Purity
  Protein G Purified
• Antibody buffer for storage
  PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Antibody s concentration
  1 mg/ml
• Antibody s specificity
  Detects ~45kDa. No cross-reactivity against Kir2.2 or Kir2.3.
• Storage recommendations
  -20ºC
• Shipping recommendations
  Blue Ice or 4ºC
• Antibody certificate of analysis
  1 µg/ml of SMC-311 was sufficient for detection of Kir2.2 in 30 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Antibody in cell
  Membrane
• Tissue specificity
  See included datasheet or contact our support service
• Scientific context
  The Kir2.1 inward-rectifier potassium ion channel is encoded by the KCNJ2 gene. A defect in this gene is associated with Andersen-Tawil syndrome (1).
• Bibliography
  1. Donaldson M.R., Yoon G., Fu Y.H., Ptacek L.J. (2004). Ann. Med. 36 Suppl 1: 92–7.
• Released date
  6-Jul-2009
• Tested applications
  To be tested
• Tested reactivity
  To be tested
• NCBI number
  NP_446433
• Gene number
  117052
• Protein number
  Q6U7SO
• Antibody s datasheet
  Contact our support service
• Representative figure link
  more | details | see here
• Representative figure legend
  Immunohistochemistry analysis using Mouse Anti-Kir2.2 Potassium Channel Monoclonal Antibody, Clone S124B-38 (SMC-311). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Kir2.2 Potassium Channel Monoclonal Antibody (SMC-311) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Western Blot analysis of Human Cell lysates showing detection of Kir2.2 Potassium Channel protein using Mouse Anti-Kir2.2 Potassium Channel Monoclonal Antibody, Clone S124B-38 (SMC-311). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Kir2.2 Potassium Channel Monoclonal Antibody (SMC-311) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Immunohistochemistry analysis using Mouse Anti-Kir2.2 Potassium Channel Monoclonal Antibody, Clone S124B-38 (SMC-311). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Kir2.2 Potassium Channel Monoclonal Antibody (SMC-311) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Mouse Anti-Kir2.2 Potassium Channel Antibody [S124B-38] used in Immunohistochemistry (IHC) on Mouse backskin (SMC-311) Mouse Anti-Kir2.2 Potassium Channel Antibody [S124B-38] used in Western Blot (WB) on Human Cell lysates (SMC-311) Mouse Anti-Kir2.2 Potassium Channel Antibody [S124B-38] used in Immunohistochemistry (IHC) on Human hippocampus (SMC-311)
• Warning information
  Non-hazardous
• Country of production
  Canada
• Total weight kg
  1.4
• Net weight g
  0.1
• Stock availability
  In Stock
• Other related products
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus, Rattus norvegicus

• Gene target
  Kir2   Monoclonal   IgG1   S124B-38  
• Gene symbol
  IGLVI-38, SNORD115-38, IGHV3-38, IGKV2D-38, IGKV2-38, IGHVIII-38-1, RNU6-38P, TPPP3, IGHV4-38-2, IGHV3-38-3
• Short name
  Mouse Anti- Kir2 2 Monoclonal IgG1, Clone S124B-38
• Technique
  anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
• Host
  mouse
• Isotype
  IgG1, IgG1
• Label
  PerCP
• Species
  Rat, Mouses, Rats
• Alternative name
  Mouse Antibody toRat Kir2.2 monoclonal IgG1, clonality S124B-38
• Alternative technique
  murine, antibodies
• Clone name
  S124B-38

Gene info

• Identity
  HGNC:5935
• Gene
  IGLVI-38
• Long gene name
  immunoglobulin lambda variable (I)-38 (pseudogene)
• Synonyms gene name
  • immunoglobulin lambda variable (I)-38
  • immunoglobulin lambda variable (I)-38 pseudogene
• Synonyms
  • IGLV(I)-38
• GenBank acession
  • D87009
• Locus
  22q11.22
• Discovery year
  2000-05-08
• Entrez gene record
  28768
• RefSeq identity
  • NG_000002
• Classification
  • Immunoglobulin lambda locus at 22q11.2
• VEGA ID
  OTTHUMG00000151073

Gene info

• Identity
  HGNC:33057
• Gene
  SNORD115-38
• Long gene name
  small nucleolar RNA, C/D box 115-38
• Synonyms
  • HBII-52-38
• GenBank acession
  • AF250841
• Locus
  15q11.2
• Discovery year
  2007-02-15
• Entrez gene record
  100033812
• Pubmed identfication
  • 11106375
  • 11726556
• RefSeq identity
  • NR_003353.1
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:5601
• Gene
  IGHV3-38
• Long gene name
  immunoglobulin heavy variable 3-38 (non-functional)
• Synonyms gene name
  • immunoglobulin heavy variable 3-38
• GenBank acession
  • M99669
• Locus
  14q32.33
• Discovery year
  2000-04-04
• Entrez gene record
  28429
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000152093

Gene info

• Identity
  HGNC:5803
• Gene
  IGKV2D-38
• Long gene name
  immunoglobulin kappa variable 2D-38 (pseudogene)
• Synonyms gene name
  • immunoglobulin kappa variable 2D-38
  • immunoglobulin kappa variable 2D-38 pseudogene
• GenBank acession
  • X71892
• Locus
  2p11.2
• Discovery year
  2000-04-18
• Entrez gene record
  28879
• RefSeq identity
  • NG_000833
• Classification
  • Immunoglobulin kappa locus at 2p11.2
• VEGA ID
  OTTHUMG00000159961

Gene info

• Identity
  HGNC:5787
• Gene
  IGKV2-38
• Long gene name
  immunoglobulin kappa variable 2-38 (pseudogene)
• Synonyms gene name
  • immunoglobulin kappa variable 2-38
  • immunoglobulin kappa variable 2-38 pseudogene
• GenBank acession
  • X71888
• Locus
  2p11.2
• Discovery year
  2000-04-18
• Entrez gene record
  28917
• RefSeq identity
  • NG_000834
• Classification
  • Immunoglobulin kappa locus at 2p11.2
• VEGA ID
  OTTHUMG00000151685

Gene info

• Identity
  HGNC:5699
• Gene
  IGHVIII-38-1
• Long gene name
  immunoglobulin heavy variable (III)-38-1 (pseudogene)
• Synonyms gene name
  • immunoglobulin heavy variable (III)-38-1
  • immunoglobulin heavy variable (III)-38-1 pseudogene
• Synonyms
  • IGHV(III)-38-1
• GenBank acession
  • AB019439
• Locus
  14q32.33
• Discovery year
  2000-04-17
• Entrez gene record
  28346
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000152090

Gene info

• Identity
  HGNC:34282
• Gene
  RNU6-38P
• Long gene name
  RNA, U6 small nuclear 38, pseudogene
• Synonyms gene
  • RNU6-38
• Synonyms gene name
  • RNA, U6 small nuclear 38
• Locus
  13q22.2
• Discovery year
  2008-06-10
• Entrez gene record
  106480709

Gene info

• Identity
  HGNC:24162
• Gene
  TPPP3
• Long gene name
  tubulin polymerization promoting protein family member 3
• Synonyms
  • CGI-38
  • p25gamma
  • p20
• GenBank acession
  • BC000691
• Locus
  16q22.1
• Discovery year
  2007-05-02
• Entrez gene record
  51673
• Pubmed identfication
  • 15590652
  • 17105200
• RefSeq identity
  • NM_015964
• Classification
  • Tubulin polymerization promoting proteins
• VEGA ID
  OTTHUMG00000137516

Gene info

• Identity
  HGNC:5657
• Gene
  IGHV4-38-2
• Long gene name
  immunoglobulin heavy variable 4-38-2
• Synonyms gene
  • IGHV4-B
• Synonyms gene name
  • immunoglobulin heavy variable 4-B (provisional)
• GenBank acession
  • Z12367
• Locus
  14q32.33 not on reference assembly
• Discovery year
  2000-04-04
• Entrez gene record
  28389
• Classification
  • Immunoglobulin heavy locus at 14q32.33

Gene info

• Identity
  HGNC:5629
• Gene
  IGHV3-38-3
• Long gene name
  immunoglobulin heavy variable 3-38-3 (non-functional)
• Synonyms gene
  • IGHV3-D
• Synonyms gene name
  • immunoglobulin heavy variable 3-D (provisional)
• Synonyms
  • IGHV3-d
• GenBank acession
  • KF698732
• Locus
  14q32.33 not on reference assembly
• Discovery year
  2000-04-04
• Entrez gene record
  28404
• Pubmed identfication
  • 23541343
• Classification
  • Immunoglobulin heavy locus at 14q32.33

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

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