Mouse Anti-Rat HCN2 Monoclonal IgG1, Clone S71-37
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Catalog numberSMC-305D-APC
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PricePlease ask
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Size100 µg
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CloneS71-37
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ImmunogenFusion protein amino acids 761-863 of rat HCN2
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Antibody s full descriptionMouse Anti-Rat HCN2 Monoclonal IgG1 Antibody, Clone: S71-37: APC
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameBCNG2 Antibody, HAC1 Antibody, brain cyclic nucleotide gated channel 2 Antibody, Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel Antibody
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Raised inMouse
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Antibody s targetHCN2
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Primary research fieldsNeuroscience, Ion Channels, Cyclic Nucleotide-Gated Ion Channels, cardiovascular System, Heart
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BrandnameNone
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Antibodies applicationsWB, IHC, ICC/IF, IP, AM
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Antibody s reactivityHuman, Mouse, Rat
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Antibody s dilutionsWB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~95kDa. No cross-reactivity against HCN1.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysis1 µg/ml of SMC-305 was sufficient for detection of HCN2 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellMembrane
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextHyperpolarization-activated cyclic nucleotide-gated ion channel 2 (HCN2) is an integral membrane protein that helps establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). Ion channels are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligand- gated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels; each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inward-rectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2). Specifically, hyperpolarization-activated cation channels of the HCN gene family contribute to spontaneous rhythmic activity in both the heart and brain (3).
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Bibliography1. Hille B. (2001) Ion Channels of Excitable Membranes, 3rd Ed., Sinauer Associated Inc.:Sunderland, MA USA. 2. www.iochannels.org 3. Zong X., et al. (2005) J Biol Chem. 280(40): 34224-34232
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Released date6-Jul-2009
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_446136.1
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Gene number114244
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Protein numberQ9JKA9
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunohistochemistry analysis using Mouse Anti-HCN2 Monoclonal Antibody, Clone S71-37 (SMC-305). Tissue: frozen brain section. Species: mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN2 Monoclonal Antibody (SMC-305) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT. Western Blot analysis of Rat brain membrane lysate showing detection of HCN2 protein using Mouse Anti-HCN2 Monoclonal Antibody, Clone S71-37 (SMC-305). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HCN2 Monoclonal Antibody (SMC-305) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Immunohistochemistry analysis using Mouse Anti-HCN2 Monoclonal Antibody, Clone S71-37 (SMC-305). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-HCN2 Monoclonal Antibody (SMC-305) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Mouse Anti-HCN2 antibody [S71-37] used in Immunohistochemistry (IHC) on mouse frozen brain section (SMC-305) Mouse Anti-HCN2 Antibody [S71-37] used in Western Blot (WB) on Rat brain membrane lysate (SMC-305) Mouse Anti-HCN2 Antibody [S71-37] used in Immunohistochemistry (IHC) on Human hippocampus (SMC-305)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus, Rattus norvegicus
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Gene target
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Gene symbolSNORD115-37, IGLV5-37, IGHV3-37, IGKV1-37, IGKV1D-37, RNU6-37P, HCN2, LIN37, RNVU1-14, NIP7
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Short nameMouse Anti- HCN2 Monoclonal IgG1, Clone S71-37
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Techniqueanti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG1, IgG1
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LabelAPC
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SpeciesRat, Mouses, Rats
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Alternative nameMouse Antibody toRat HCN2 monoclonal IgG1, clonality S71-37
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Alternative techniquemurine, antibodies
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Clone nameS71-37
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Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 115-37
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin lambda variable 5-37
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GenBank acession
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Locus
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Discovery year2000-05-08
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin lambda locus at 22q11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 3-37 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable 3-37
- immunoglobulin heavy variable 3-37 pseudogene
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 1-37 (non-functional)
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Synonyms gene name
- immunoglobulin kappa variable 1-37
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 1D-37 (non-functional)
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Synonyms gene name
- immunoglobulin kappa variable 1D-37
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameRNA, U6 small nuclear 37, pseudogene
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Synonyms gene
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Synonyms gene name
- RNA, U6 small nuclear 37
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Locus
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Discovery year2008-06-10
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Entrez gene record
Gene info
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Identity
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Gene
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Long gene namehyperpolarization activated cyclic nucleotide gated potassium and sodium channel 2
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Synonyms gene
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Synonyms gene name
- hyperpolarization activated cyclic nucleotide-gated potassium channel 2
- hyperpolarization activated cyclic nucleotide gated potassium channel 2
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-08-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Cyclic nucleotide gated channels
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namelin-37 DREAM MuvB core complex component
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Synonyms gene name
- lin-37 homolog (C. elegans)
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-05-17
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameRNA, variant U1 small nuclear 14
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Synonyms
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Locus
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Discovery year2013-04-25
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Variant U1 small nuclear RNAs
Gene info
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Identity
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Gene
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Long gene namenucleolar pre-rRNA processing protein NIP7
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Synonyms gene name
- nuclear import 7 homolog (S. cerevisiae)
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Synonyms
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GenBank acession
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Locus
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Discovery year2005-12-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Ribosomal biogenesis factors
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
