Mouse Anti-Rat GIT1 Monoclonal IgG1, Clone S39B-8
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Catalog numberSMC-413D-STR
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PricePlease ask
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Size100 µg
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CloneS39B-8
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ImmunogenFusion protein amino acids 375-770 (C-terminus) of rat GIT1
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Antibody s full descriptionMouse Anti-Rat GIT1 Monoclonal IgG1 Antibody, Clone: S39B-8: Streptavidin
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameARF GAP GIT1 Antibody, ARF GTPase-activating proteinAntibody, CAT1 Antibody, GRK-interacting protein 1 Antibody, ARF GTPase-activating protein GIT1 Antibody, Cool-associated and tyrosine-phosphorylated protein 1 Antibody, G protein-coupled receptor kinase-interactor 1 Antibody, GIT 1 Antibody
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Raised inMouse
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Antibody s targetGIT1
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Primary research fieldsNeuroscience, Cell Structure, Cytoplasmic Markers, Cell Signaling, Scaffolds, Post-translational Modifications, Phosphorylation
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BrandnameNone
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Antibodies applicationsWB, ICC/IF, IP
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Antibody s reactivityHuman, Mouse, Rat
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Antibody s dilutionsWB (1:1000); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~90kDa. Does not cross-react with GIT2.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysis1 µg/ml of SMC-413 was sufficient for detection of GIT1 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellCytoplasm
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextG-protein coupled receptor (GPCR) kinase interacting proteins 1 and 2 (GIT-1 and GIT-2) are highly conserved, ubiquitous scaffold proteins involved in localized signaling to help regulate focal contact assembly and cytoskeletal dynamics. GIT proteins contain multiple interaction domains that allow interaction with small GTPases (including ARF, Rac and cdc42), kinases (such as PAK and MEK), the Rho family GEF PIX, and the focal adhesion protein paxillin (reviewed in 1). GIT-1 is localized to focal adhesions, cytoplasmic complexes and membrane protrusions, and regulates cell protrusion formation and cell migration (2). GIT-1 has also been implicated in neuronal functions including synapse formation (3) and the pathology of Huntington disease (4). Huntington disease is a genetic neurodegenerative condition involving a mutation in the huntington gene. The huntington gene product (htt) is ubiquitinated and degraded in human Huntington disease brains (5). Htt interacts directly with GIT-1 causing enhanced htt proteolysis, indicating that GIT-1 distribution and function may contribute to Huntington disease pathology (4).
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Bibliography1. Hoefen R.J. and Berk B.C. (2006) J. Cell Sci. 119: 146 1475. 2. Manabe R., et al. (2002) J. Cell Sci. 115: 1497-1510. 3. Zhang H., et al. (2003) J. Cell Biol. 161: 131-142. 4. Goehler H., et al. (2004) Mol. Cell 15: 853-865. 5. Mende-Mueller L.M., et al. (2001) J. Neurosci. 21: 1830-1837.
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Released date20-Feb-2013
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_1140021.1
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Gene number83709
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Protein numberQ9Z272.1
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-GIT1 Monoclonal Antibody, Clone S39B-8 (SMC-413). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GIT1 Monoclonal Antibody (SMC-413) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GIT1 Antibody (D) Composite. Western Blot analysis of Rat brain membrane lysate showing detection of GIT1 protein using Mouse Anti-GIT1 Monoclonal Antibody, Clone S39B-8 (SMC-413). Primary Antibody: Mouse Anti-GIT1 Monoclonal Antibody (SMC-413) at 1:1000. Mouse Anti-GIT1 Antibody [S39B-8] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-413) Mouse Anti-GIT1 Antibody [S39B-8] used in Western Blot (WB) on Rat brain membrane lysate (SMC-413)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus, Rattus norvegicus
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Gene target
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Gene symbolGIT1, SNORD114-8, RNU6-8, SNORD113-8, SNORD116-8, IGKV2OR2-8, MIR1302-8, PIRC35
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Short nameMouse Anti- GIT1 Monoclonal IgG1, Clone S39B-8
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Techniqueanti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG1, IgG1
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LabelStreptavidin
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SpeciesRat, Mouses, Rats
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Alternative nameMouse Antibody toRat GIT1 monoclonal IgG1, clonality S39B-8
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Alternative techniquemurine, antibodies
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Clone nameS39B-8
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Gene info
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Identity
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Gene
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Long gene nameGIT ArfGAP 1
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Synonyms gene name
- G protein-coupled receptor kinase interactor 1
- G protein-coupled receptor kinase interacting ArfGAP 1
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GenBank acession
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Locus
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Discovery year2000-07-31
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- ArfGAPs
- Ankyrin repeat domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 114-8
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Synonyms
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Locus
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Discovery year2006-09-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameRNA, U6 small nuclear 8
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Synonyms
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Locus
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Discovery year2008-06-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nuclear RNAs
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 113-8
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Synonyms
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Locus
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Discovery year2006-09-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 116-8
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 2/OR2-8 (pseudogene)
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Synonyms gene name
- immunoglobulin kappa variable 2/OR2-8
- immunoglobulin kappa variable 2/OR2-8 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa (IGK) orphons
Gene info
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Identity
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Gene
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Long gene namemicroRNA 1302-8
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Synonyms gene
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Synonyms
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Locus
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Discovery year2008-11-03
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR1302 family
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 35
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
