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Clone
S38-8
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Immunogen
Fusion protein amino acids 2025-2161 of rat Cav1.3
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Antibody s full description
Mouse Anti-Rat Cav1.3 Monoclonal IgG1 Antibody, Clone: S38-8: Alkaline Phosphatase
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Antibody s category
Monoclonal Antibodies
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Antibody s other name
alpha-1 polypeptide Antibody, CAC1D_HUMAN Antibody, CACH3 Antibody, CACN4 Antibody, CACNA 1D Antibody, Cacna1d Antibody, CACNL1A2 Antibody, Calcium channel Antibody, Calcium channel L type alpha 1 polypeptide isoform 2 Antibody, Calcium channel neuroendocrine/brain type alpha 1 subunit Antibody, Calcium channel voltage dependent L type alpha 1D subunit Antibody, CCHL1A2 Antibody, isoform 2 Antibody, L type Antibody, Voltage dependent L type calcium channel subunit alpha 1D Antibody, Voltage gated calcium channel alpha 1 subunit Antibody, Voltage gated calcium channel alpha subunit Cav1.3 Antibody, Voltage gated calcium channel subunit alpha Cav1.3 Antibody, Voltage-dependent L-type calcium channel subunit alpha-1D Antibody, Voltage-gated calcium channel subunit alpha Cav1.3 Antibody
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Raised in
Mouse
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Antibody s target
Cav1.3
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Primary research fields
Neuroscience, Ion Channels, Calcium Channels, Voltage-Gated Calcium Channels, Cancer, Cell Signaling
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Brandname
None
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Antibodies applications
WB, IHC, ICC/IF, IP , AM
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Antibody s reactivity
Human, Mouse, Rat
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Antibody s dilutions
WB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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Purity
Protein G Purified
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Antibody buffer for storage
PBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects ~250kDa. No cross-reactivity against Cav1.2.
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Storage recommendations
-20ºC
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Shipping recommendations
Blue Ice or 4ºC
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Antibody certificate of analysis
1 µg/ml of SMC-302 was sufficient for detection of Cav1.3 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cell
Membrane , Cell Membrane
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Tissue specificity
See included datasheet or contact our support service
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Scientific context
CaV1.3, also known as the calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), is a human gene. CaV1.3 subunits are primarily expressed in neurons and neuroendocine cells. Some studies suggest however that CaV1.3 is also found in the atria, and may figure prominently in atrial arrhythmias (1). CaV1.3 also carries the primary sensory receptors of the mammalian cochlea, and are also expressed in the electromotile outer hair cells (2).
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Bibliography
1. Zhang Z., et al. (2005) Circulation 112: 1936-1944. 2. Johnson S.L. and Marcotti W. (2008) The Journal of Physiology. 586: 1029-1042.
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Released date
31-Jul-2009
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Tested applications
To be tested
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Tested reactivity
To be tested
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NCBI number
NP_058994.1
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Gene number
29716
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Protein number
P27732
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Antibody s datasheet
Contact our support service
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Representative figure link
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Representative figure legend
Immunohistochemistry analysis using Mouse Anti-CaV1.3 Calcium channel Monoclonal Antibody, Clone S38-8 (SMC-302). Tissue: frozen brain section. Species: mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-CaV1.3 Calcium channel Monoclonal Antibody (SMC-302) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT. Western Blot analysis of Rat showing detection of ~250 kDa Cav1.3 protein using Mouse Anti-Cav1.3 Monoclonal Antibody, Clone S38-8 (SMC-302). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Tissue cell lysate. Load: 20 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Cav1.3 Monoclonal Antibody (SMC-302) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:100 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~250 kDa. Immunohistochemistry analysis using Mouse Anti-CaV1.3 Calcium Channel Monoclonal Antibody, Clone S38-8 (SMC-302). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-CaV1.3 Calcium Channel Monoclonal Antibody (SMC-302) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Mouse Anti-CaV1.3 Calcium channel antibody [S38-8] used in Immunohistochemistry (IHC) on mouse frozen brain section (SMC-302) Mouse Anti-Cav1.3 Antibody [S38-8] used in Western Blot (WB) on Rat (SMC-302) Mouse Anti-CaV1.3 Calcium Channel Antibody [S38-8] used in Immunohistochemistry (IHC) on Human hippocampus (SMC-302)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availability
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus, Rattus norvegicus
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Gene target
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Gene symbol
SNORD114-8, RNU6-8, SNORD113-8, SNORD116-8, IGKV2OR2-8, MIR1302-8, PIRC35
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Short name
Mouse Anti- Cav1 3 Monoclonal IgG1, Clone S38-8
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Technique
anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
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Host
mouse
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Isotype
IgG1, IgG1
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Label
Alkaline Phosphatase
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Species
Rat, Mouses, Rats
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Alternative name
Mouse Antibody toRat caveolin 1, caveolae protein, 22kDa.3 monoclonal IgG1, clonality S38-8
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Alternative technique
murine, antibodies
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Alternative to gene target
caveolin 1, caveolae protein, 22kDa, BSCL3 and CGL3 and LCCNS and MSTP085 and PPH3 and VIP21, CAV1 and IDBG-37314 and ENSG00000105974 and 857, nitric-oxide synthase binding, Cell surfaces, Cav1 and IDBG-129000 and ENSMUSG00000007655 and 12389, CAV1 and IDBG-632834 and ENSBTAG00000017869 and 281040
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Clone name
S38-8
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