Mouse Anti- Phosphothreonine Monoclonal IgG1 Kappa, Clone 18F6
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Catalog numberSMC-158D-A655
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Price:
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Size100 µl
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Clone18F6
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ImmunogenPhosphothreonine conjugated to KLH
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Antibody s full descriptionMouse Anti- Phosphothreonine Monoclonal IgG1 Kappa Antibody, Clone: 18F6: ATTO 655
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Antibody s categoryMonoclonal Antibodies
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Antibody s other namePhosphoThreonine (pT) Antibody, PhosphoThreonine (pT) Antibody
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Raised inMouse
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Antibody s targetPhosphothreonine
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Primary research fieldsCell Signaling, Post-translational Modifications, Phosphorylation
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BrandnameNone
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Antibodies applicationsWB, ICC/IF, ELISA
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Antibody s reactivitySpecies Independent
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Antibody s dilutionsWB (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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PurityAscites
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Antibody buffer for storageAscites, 0.02% sodium azide
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Antibody s specificityReacts with phosphothreonine, and detects the presence of phosphothreonine in proteins of both un-stimulated and stimulated cell lysates. Does not cross-react with phosphoserine or phosphotyrosine.
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Storage recommendations4ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody in cellSee included datasheet or contact our support service
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextProtein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein’s activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine; others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time (1). Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (2-4).
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Bibliography1. Goto H. et al. (2005) Nature Cell Biology 8: 180-187. 2. Blume-Jensen P. and Hunter T. (2001) Nature 411:355-365. 3. Downward J. (2001) Nature 411: 759-762. 4. Pawson T. and Saxton T.M. (1999) Cell 97: 675-678. 5. Frackelton A.R. Jr., Ross A.H., and Eisen H.N. (1983) Mol Cell Biol. 3: 1343-1352. 6. Ross A.H., Baltimore D., and Eisen H.N. (1981) Nature 294: 654-656. 7. Ostrovsky PC. (1995) Genes Dev. 9(16): 2034-2041.
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Released date1-Apr-2008
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberRefer to NCBI
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Gene numberRefer to GenBank
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Protein numberRefer to Swiss-Prot
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-Phosphothreonine Monoclonal Antibody, Clone 18F9 (SMC-158). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Phosphothreonine Monoclonal Antibody (SMC-158) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Diffuse nuclear and cytoplasmic staining. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Phosphothreonine Antibody. (C) Composite. Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Phosphothreonine Monoclonal Antibody, Clone 18F9 (SMC-158). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Phosphothreonine Monoclonal Antibody (SMC-158) at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Mouse (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Diffuse nuclear and cytoplasmic staining. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Phosphothreonine Antibody. (C) Composite. Mouse Anti-Phosphothreonine Antibody [18F9] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SMC-158) Mouse Anti-Phosphothreonine Antibody [18F9] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SMC-158)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Short nameMouse Anti- Phosphothreonine Monoclonal IgG1 Kappa, Clone 18F6
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TechniqueMouse, anti, antibody to, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG1 Kappa, IgG1
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LabelATTO 655
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SpeciesMouse, Mouses
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Alternative nameMouse antibody to- Phosphothreonine monoclonal IgG1 Kappa, clonality 18F6
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Alternative techniquemurine, antibodies
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Clone name18F6
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data