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Clone
18F6
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Immunogen
Phosphothreonine conjugated to KLH
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Antibody s full description
Mouse Anti- Phosphothreonine Monoclonal IgG1 Kappa Antibody, Clone: 18F6: Unconjugated
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Antibody s category
Monoclonal Antibodies
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Antibody s other name
PhosphoThreonine (pT) Antibody, PhosphoThreonine (pT) Antibody
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Raised in
Mouse
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Antibody s target
Phosphothreonine
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Primary research fields
Cell Signaling, Post-translational Modifications, Phosphorylation
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Brandname
None
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Antibodies applications
WB, ICC/IF, ELISA
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Antibody s reactivity
Species Independent
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Antibody s dilutions
WB (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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Purity
Ascites
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Antibody buffer for storage
Ascites, 0.02% sodium azide
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Antibody s specificity
Reacts with phosphothreonine, and detects the presence of phosphothreonine in proteins of both un-stimulated and stimulated cell lysates. Does not cross-react with phosphoserine or phosphotyrosine.
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Storage recommendations
-20ºC
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Shipping recommendations
Blue Ice or 4ºC
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Antibody in cell
See included datasheet or contact our support service
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Tissue specificity
See included datasheet or contact our support service
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Scientific context
Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein’s activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine; others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time (1). Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (2-4).
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Bibliography
1. Goto H. et al. (2005) Nature Cell Biology 8: 180-187. 2. Blume-Jensen P. and Hunter T. (2001) Nature 411:355-365. 3. Downward J. (2001) Nature 411: 759-762. 4. Pawson T. and Saxton T.M. (1999) Cell 97: 675-678. 5. Frackelton A.R. Jr., Ross A.H., and Eisen H.N. (1983) Mol Cell Biol. 3: 1343-1352. 6. Ross A.H., Baltimore D., and Eisen H.N. (1981) Nature 294: 654-656. 7. Ostrovsky PC. (1995) Genes Dev. 9(16): 2034-2041.
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Released date
1-Apr-2008
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Tested applications
To be tested
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Tested reactivity
To be tested
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NCBI number
Refer to NCBI
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Gene number
Refer to GenBank
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Protein number
Refer to Swiss-Prot
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Antibody s datasheet
Contact our support service
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Representative figure link
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Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Phosphothreonine Monoclonal Antibody, Clone 18F9 (SMC-158). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Phosphothreonine Monoclonal Antibody (SMC-158) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Diffuse nuclear and cytoplasmic staining. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Phosphothreonine Antibody. (C) Composite. Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Phosphothreonine Monoclonal Antibody, Clone 18F9 (SMC-158). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Phosphothreonine Monoclonal Antibody (SMC-158) at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Mouse (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Diffuse nuclear and cytoplasmic staining. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Phosphothreonine Antibody. (C) Composite. Mouse Anti-Phosphothreonine Antibody [18F9] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SMC-158) Mouse Anti-Phosphothreonine Antibody [18F9] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SMC-158)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availability
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus