-
Clone
11G1
-
Immunogen
Synthetic N-terminal arginylated peptide conjugated to KLH
-
Antibody s full description
Mouse Anti- N-terminal Arginylation Monoclonal IgG3 Antibody, Clone: 11G1: ATTO 390
-
Antibody s category
Monoclonal Antibodies
-
Antibody s other name
N-terminal Arginine Antibody, N-terminal Arginylation Antibody, N-terminal Arginylated Antibody, N terminal Arginine Antibody, N terminal Arginylation Antibody, N terminal Arginylated Antibody, Amino-terminal Arginine Antibody, Amino-terminal Arginylation Antibody, Amino-terminal Arginylated Antibody, Amino terminal Arginine Antibody, Amino terminal Arginylation Antibody, Amino terminal Arginylated Antibody
-
Raised in
Mouse
-
Antibody s target
N-terminal Arginylation
-
Primary research fields
Cell Signaling, Post Translational Modifications, Arginylation
-
Brandname
None
-
Antibodies applications
WB
-
Antibody s reactivity
Species Independent
-
Antibody s dilutions
WB (1:1000); optimal dilutions for assays should be determined by the user.
-
Purity
Protein G Purified
-
Antibody buffer for storage
PBS pH 7.4, 50% glycerol, 0.9% Sodium Azide
-
Antibody s concentration
1 mg/ml
-
Antibody s specificity
Specific for N-terminal arginine, next to both glutamic acid and aspartic acid. Does not detect internal arginine.
-
Storage recommendations
-20ºC
-
Shipping recommendations
Blue Ice or 4ºC
-
Antibody certificate of analysis
A 1:1000 dilution of SMC-265 was sufficient for detection of N-terminal Arginylation in 0.5 ug of N-terminal Arginine peptide conjugated to BSA by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
-
Antibody in cell
Endoplasmic Reticulum
-
Tissue specificity
See included datasheet or contact our support service
-
Scientific context
Protein arginylation is the post-translational addition of arginine to proteins by arginyltransferase ATE1. Arginylation of proteins has been found to play an essential role in physiological pathways during embryogenesis and adulthood (1). Arginylation has also been shown to regulate cell stress responses, including ER stress, cytosolic misfolded proteins, and heat stress (2).
-
Bibliography
1. Saha S. and Kashina A. (2011) Dev Biol. 385(1): 1-8. 2. Deka K., et al. (2016) Cell Death Discov. 2: 16074.
-
Released date
23-Feb-2017
-
Tested applications
To be tested
-
Tested reactivity
To be tested
-
NCBI number
Refer to NCBI
-
Gene number
Refer to GenBank
-
Protein number
Refer to Swiss-Prot
-
Antibody s datasheet
Contact our support service
-
-
Representative figure legend
Western Blot analysis of N-terminal Arginine-BSA showing detection of 67 kDa N-terminal Arginylation protein using Mouse Anti-N-terminal Arginylation Monoclonal Antibody, Clone 11G1 (SMC-265). Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: RDHKH-BSA. Lane 4: REHKH-BSA. Lane 5: HKH-BSA. Lane 6: HKERD-BSA. Lane 7: HKRRE-BSA. Load: 0.5 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-N-terminal Arginylation Monoclonal Antibody (SMC-265) at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 2 hour at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 67 kDa. Other Band(s): 75kDa RDHKH-BSA, and REHKH-BSA. Mouse Anti-N-terminal Arginylation Antibody [11G1] used in Western Blot (WB) on N-terminal Arginine-BSA (SMC-265)
-
Warning information
Non-hazardous
-
Country of production
Canada
-
Total weight kg
1.4
-
Net weight g
0.1
-
Stock availability
In Stock
-
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
-
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Latin name
Mus musculus