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Mouse Anti-N-terminal Arginylation Monoclonal IgG3, Clone 11G1

  • Catalog number
    SMC-265D-A700
  • Product name
    Mouse Anti-N-terminal Arginylation Monoclonal IgG3, Clone 11G1
  • Size
    100 µg
  • Label
    ATTO 700
  • Clone
    11G1
  • Immunogen
    Synthetic N-terminal arginylated peptide conjugated to KLH
  • Antibody s full description
    Mouse Anti- N-terminal Arginylation Monoclonal IgG3 Antibody, Clone: 11G1: ATTO 700
  • Antibody s category
    Monoclonal Antibodies
  • Antibody s other name
    N-terminal Arginine Antibody, N-terminal Arginylation Antibody, N-terminal Arginylated Antibody, N terminal Arginine Antibody, N terminal Arginylation Antibody, N terminal Arginylated Antibody, Amino-terminal Arginine Antibody, Amino-terminal Arginylation Antibody, Amino-terminal Arginylated Antibody, Amino terminal Arginine Antibody, Amino terminal Arginylation Antibody, Amino terminal Arginylated Antibody
  • Raised in
    Mouse
  • Isotype
    IgG3, IgG3
  • Antibody s target
    N-terminal Arginylation
  • Primary research fields
    Cell Signaling, Post Translational Modifications, Arginylation
  • Brandname
    None
  • Antibodies applications
    WB
  • Antibody s reactivity
    Species Independent
  • Antibody s dilutions
    WB (1:1000); optimal dilutions for assays should be determined by the user.
  • Purity
    Protein G Purified
  • Antibody buffer for storage
    PBS pH 7.4, 50% glycerol, 0.9% Sodium Azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Specific for N-terminal arginine, next to both glutamic acid and aspartic acid. Does not detect internal arginine.
  • Storage recommendations
    -20ºC
  • Shipping recommendations
    Blue Ice or 4ºC
  • Antibody certificate of analysis
    A 1:1000 dilution of SMC-265 was sufficient for detection of N-terminal Arginylation in 0.5 ug of N-terminal Arginine peptide conjugated to BSA by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
  • Antibody in cell
    Endoplasmic Reticulum
  • Tissue specificity
    See included datasheet or contact our support service
  • Scientific context
    Protein arginylation is the post-translational addition of arginine to proteins by arginyltransferase ATE1. Arginylation of proteins has been found to play an essential role in physiological pathways during embryogenesis and adulthood (1). Arginylation has also been shown to regulate cell stress responses, including ER stress, cytosolic misfolded proteins, and heat stress (2).
  • Bibliography
    1. Saha S. and Kashina A. (2011) Dev Biol. 385(1): 1-8. 2. Deka K., et al. (2016) Cell Death Discov. 2: 16074.
  • Released date
    23-Feb-2017
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • NCBI number
    Refer to NCBI
  • Gene number
    Refer to GenBank
  • Protein number
    Refer to Swiss-Prot
  • Antibody s datasheet
    Contact our support service
  • Representative figure link
    Representative_figure_link
  • Representative figure legend
    Western Blot analysis of N-terminal Arginine-BSA showing detection of 67 kDa N-terminal Arginylation protein using Mouse Anti-N-terminal Arginylation Monoclonal Antibody, Clone 11G1 (SMC-265). Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: RDHKH-BSA. Lane 4: REHKH-BSA. Lane 5: HKH-BSA. Lane 6: HKERD-BSA. Lane 7: HKRRE-BSA. Load: 0.5 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-N-terminal Arginylation Monoclonal Antibody (SMC-265) at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 2 hour at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 67 kDa. Other Band(s): 75kDa RDHKH-BSA, and REHKH-BSA. Mouse Anti-N-terminal Arginylation Antibody [11G1] used in Western Blot (WB) on N-terminal Arginine-BSA (SMC-265)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availability
    In Stock
  • Other related products
    Other_related_products
  • Gene target
    - Arginylation Monoclonal IgG3 11G1
  • Host
    mouse
  • isotype filter
    • IgG3
  • Short name
    Mouse Anti-N-terminal Arginylation Monoclonal IgG3, Clone 11G1
  • label filter
    • ATTO 700
  • Clone name
    11G1
  • technique filter
    • anti-
    • Mouse
    • anti
  • Technique
    anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
  • Alternative technique
    murine, antibodies