Mouse Anti-Mouse GABA-A Receptor Beta1 Monoclonal IgG1, Clone S96-55
-
Catalog numberSMC-340D-A655
-
PricePlease ask
-
Size100 µg
-
-
CloneS96-55
-
ImmunogenFusion protein amino acids 327-450 of mouse GABA-A-R-Beta1
-
Antibody s full descriptionMouse Anti-Mouse GABA A Receptor Monoclonal IgG1 Antibody, Clone: S96-55: ATTO 655
-
Antibody s categoryMonoclonal Antibodies
-
Antibody s other nameAW061132 antibody, B230208N19Rik antibody, GABA(A) receptor beta 1 antibody, GABA(A) receptor subunit beta-1 antibody, GABA-A receptor beta-1 polypeptide antibody, Gabrb-1 antibody, GABRB1 antibody, Gamma aminobutyric acid (GABA) A receptor beta 1 antibody, Gamma Aminobutyric Acid A Receptor Beta 1 antibody, Gamma Aminobutyric Acid Receptor beta-1 antibody, Gamma-aminobutyric acid (GABA) A receptor subunit beta 1 antibody, Gamma-aminobutyric acid receptor subunit beta-1 antibody, GARB1 antibody, GBRB1_HUMAN antibody
-
Raised inMouse
-
Antibody s targetGABA A Receptor
-
Primary research fieldsNeuroscience, Neurotransmitter Receptors, GABA Receptors, GABAA Receptors
-
BrandnameNone
-
Antibodies applicationsWB, IHC, ICC/IF , AM
-
Antibody s reactivityHuman, Mouse, Rat
-
Antibody s dilutionsWB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
-
PurityProtein G Purified
-
Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
-
Antibody s concentration1 mg/ml
-
Antibody s specificityDetects ~55kDa. No cross-reactivity against GABA-A-R-Beta 2 or –Beta3.
-
Storage recommendations-20ºC
-
Shipping recommendationsBlue Ice or 4ºC
-
Antibody certificate of analysis2 µg/ml of SMC-340 was sufficient for detection of Beta1 GABA receptor in 10 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
-
Antibody in cellCell Membrane , Cell Junction , Synapse , Postsynaptic Cell Membrane
-
Tissue specificitySee included datasheet or contact our support service
-
Scientific contextThe GABA-A receptor is a member of the superfamily of fast acting ligand-gated ion channels. The individual subunits of these receptors have similar sequences and structural features (1). GABA-A receptors are the major fast inhibitory neurotransmitter gated ion channels in the brain (2).
-
Bibliography1. Bracamontes J.R. and Steinbach J.H. (2008) J Bio Chem. 283: 26128-26136. 2. Macdonald R.L., Olsen R.W. (1993) Annu Rev Neurosci. 17: 569-602.
-
Released date13-Apr-2010
-
Tested applicationsTo be tested
-
Tested reactivityTo be tested
-
NCBI numberNP_032095.1
-
Gene number14400
-
Protein numberP50571
-
Antibody s datasheetContact our support service
-
Representative figure link
-
Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone S96-55 (SMC-340). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody (SMC-340) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Positive. Western Blot analysis of Human Cell line lysates showing detection of GABA A Receptor protein using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone S96-55 (SMC-340). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody (SMC-340) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Immunohistochemistry analysis using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone S96-55 (SMC-340). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody (SMC-340) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Dermal staining. Mouse Anti-GABA A Receptor Antibody [S96-55] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HaCaT cells (SMC-340) Mouse Anti-GABA A Receptor Antibody [S96-55] used in Western Blot (WB) on Human Cell line lysates (SMC-340) Mouse Anti-GABA A Receptor Antibody [S96-55] used in Immunohistochemistry (IHC) on Mouse backskin (SMC-340)
-
Warning informationNon-hazardous
-
Country of productionCanada
-
Total weight kg1.4
-
Net weight g0.1
-
Stock availabilityIn Stock
-
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
-
AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
-
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Latin nameMus musculus
-
Gene target
-
Gene symbolIGHV4-55, IGLV11-55, SNORD110, ABAT, HLA-A, RNU6-55P, NEK1, RCN2, IGHV5-10-1, CIDEA
-
Short nameMouse Anti-Mouse GABA-A Receptor Beta1 Monoclonal IgG1, Clone S96-55
-
Techniqueanti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
-
Hostmouse
-
IsotypeIgG1, IgG1
-
LabelATTO 655
-
SpeciesMouse, Mouses
-
Alternative nameMouse Antibody toMouse GABA-A Receptor Beta1 monoclonal IgG1, clonality S96-55
-
Alternative techniquemurine, antibodies
-
Clone nameS96-55
-
Gene info
-
Identity
-
Gene
-
Long gene nameimmunoglobulin heavy variable 4-55 (pseudogene)
-
Synonyms gene name
- immunoglobulin heavy variable 4-55
- immunoglobulin heavy variable 4-55 pseudogene
-
GenBank acession
-
Locus
-
Discovery year2000-04-04
-
Entrez gene record
-
RefSeq identity
-
Classification
- Immunoglobulin heavy locus at 14q32.33
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameimmunoglobulin lambda variable 11-55 (non-functional)
-
Synonyms gene name
- immunoglobulin lambda variable 11-55
-
GenBank acession
-
Locus
-
Discovery year2000-05-08
-
Entrez gene record
-
RefSeq identity
-
Classification
- Immunoglobulin lambda locus at 22q11.2
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namesmall nucleolar RNA, C/D box 110
-
Synonyms
-
Locus
-
Discovery year2006-05-08
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Small nucleolar RNAs, C/D box
Gene info
-
Identity
-
Gene
-
Long gene name4-aminobutyrate aminotransferase
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year1996-03-13
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namemajor histocompatibility complex, class I, A
-
GenBank acession
-
Locus
-
Discovery year2001-06-22
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- C1-set domain containing
- Histocompatibility complex
-
VEGA ID
-
Locus Specific Databases
Gene info
-
Identity
-
Gene
-
Long gene nameRNA, U6 small nuclear 55, pseudogene
-
Synonyms gene
-
Synonyms gene name
- RNA, U6 small nuclear 55
-
Locus
-
Discovery year2011-08-08
-
Entrez gene record
-
RefSeq identity
Gene info
-
Identity
-
Gene
-
Long gene nameNIMA related kinase 1
-
Synonyms gene name
- NIMA (never in mitosis gene a)-related kinase 1
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year1998-08-26
-
Entrez gene record
-
Pubmed identfication
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namereticulocalbin 2
-
Synonyms gene name
- reticulocalbin 2, EF-hand calcium binding domain
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year1997-10-10
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- CREC family
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameimmunoglobulin heavy variable 5-10-1
-
Synonyms gene
-
Synonyms gene name
- immunoglobulin heavy variable 5-A (provisional, gene/pseudogene)
- immunoglobulin heavy variable 5-A (provisional)
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2000-04-04
-
Entrez gene record
-
RefSeq identity
-
Classification
- Immunoglobulin heavy locus at 14q32.33
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namecell death inducing DFFA like effector a
-
Synonyms gene name
- cell death-inducing DFFA-like effector a
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year1998-07-15
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
VEGA ID
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data