Mouse Anti-Human GABA A Receptor Alpha5 Monoclonal IgG1, Clone S415-24
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Catalog numberSMC-494D-P594
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PricePlease ask
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Size100 µg
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CloneS415-24
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ImmunogenFusion protein amino acids 368-419 (last cytoplasmic domain before extracellular C-terminus) of human GABA A Receptor Alpha5.
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Antibody s full descriptionMouse Anti-Human GABA A Receptor Monoclonal IgG1 Antibody, Clone: S415-24: PE/ATTO 594
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameGamma-aminobutyric acid receptor subunit alpha-5 Antibody, GABA(A) Receptor subunit Alpha-5 Antibody, Gabra5 Antibody
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Raised inMouse
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Antibody s targetGABA A Receptor
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Primary research fieldsNeuroscience, Ion channels, Neurotransmitter Receptors, GABA Receptors, GABAA Receptors, Neurodegeneration, Alzheimer's Disease
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BrandnameNone
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Antibodies applicationsWB, IHC, ICC/IF
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Antibody s reactivityHuman, Mouse, Rat
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Antibody s dilutionsWB (1:1000); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.1% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~55kDa. Does not cross-react with GABA A Receptor Alpha2 or other GABA-A Receptors based on KO validation data.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysisA 1:100 dilution of SMC-494 was sufficient for detection of GABA-A R, Alpha5 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellCell Junction , Synapse , Postsynaptic Cell Membrane , Cell Membrane
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextThe GABA A Receptor is a member of the superfamily of fast acting ligand-gated ion channels. The individual subunits of these receptors have similar sequences and structural features (1). GABA-A receptors are the major fast inhibitory neurotransmitter gated ion channels in the brain (2). Recent research shows their usage in investigating side effects of drugs and the effects of learning and memory. Inverse agonists of the Alpha5 subunit may be useful in the treatment of Alzheimer's Disease (3).
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Bibliography1. Bracamontes J.R. and Steinbach J.H. (2008) J Bio Chem. 283: 26128-26136. 2. Macdonald R.L., Olsen R.W. (1993) Annu Rev Neurosci. 17: 569-602. 3. Hengen K.B., et al. (2012) PLoS One. 7(1): e30608.
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Released date10-Mar-2015
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_000801.1
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Gene number2558
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Protein numberP31644
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-A Receptor Alpha5 Monoclonal Antibody, Clone S415-24 (SMC-494). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GABA-A Receptor Alpha5 Monoclonal Antibody (SMC-494) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Junction, Synapse, Postsynaptic Cell Membrane, Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GABA-A Receptor Alpha5 Antibody (D) Composite. Western Blot analysis of Mouse Brain showing detection of ~55 kDa GABA A Receptor Alpha 5 protein using Mouse Anti-GABA A Receptor Alpha 5 Monoclonal Antibody, Clone S415-24 (SMC-494). Lane 1: MW Ladder. Lane 2: Mouse Brain. Load: 20 µg. Primary Antibody: Mouse Anti-GABA A Receptor Alpha 5 Monoclonal Antibody (SMC-494) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~55 kDa. Mouse Anti-GABA-A Receptor Alpha5 Antibody [S415-24] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-494) Mouse Anti-GABA A Receptor Alpha 5 Antibody [S415-24] used in Western Blot (WB) on Mouse Brain (SMC-494)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
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TestStressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin nameMus musculus
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Gene target
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Gene symbolSNORD115-24, SNORD116-24, IGLV3-24, SNORD114-24, ABAT, CD164, IGHV1-24, ERVW-24, IGKV2-24, IGKV2D-24
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Short nameMouse Anti- GABA A Receptor Alpha5 Monoclonal IgG1, Clone S415-24
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Techniqueanti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG1, IgG1
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LabelPE/ATTO 594
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SpeciesHuman, Humans, Mouses
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Alternative nameMouse Antibody toHuman GABA A Receptor Alpha5 monoclonal IgG1, clonality S415-24
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Alternative techniquemurine, antibodies
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Clone nameS415-24
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Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 115-24
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Synonyms
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Locus
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Discovery year2010-10-05
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 116-24
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin lambda variable 3-24 (pseudogene)
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Synonyms gene name
- immunoglobulin lambda variable 3-24
- immunoglobulin lambda variable 3-24 pseudogene
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GenBank acession
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Locus
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Discovery year2000-05-08
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin lambda locus at 22q11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 114-24
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Synonyms
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Locus
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Discovery year2006-09-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene name4-aminobutyrate aminotransferase
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1996-03-13
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameCD164 molecule
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Synonyms gene name
- CD164 antigen, sialomucin
- CD164 molecule, sialomucin
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2000-06-08
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- CD molecules
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 1-24
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameendogenous retrovirus group W member 24
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Synonyms gene name
- endogenous retrovirus group W, member 24
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GenBank acession
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Locus
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Discovery year2013-10-21
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 2-24
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 2D-24 (non-functional)
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Synonyms gene name
- immunoglobulin kappa variable 2D-24
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data