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Clone
EP10
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Immunogen
Immunoprecipitate of human brain
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Antibody s full description
Mouse Anti-Human Synaptophysin Monoclonal IgG1 Antibody, Clone: EP10: Alkaline Phosphatase
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Antibody s category
Monoclonal Antibodies
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Antibody s other name
SYPH Antibody, Sypl Antibody, Syn p38 Antibody, Synaptophysin Antibody, Major synaptic vesicle protein p38 Antibody
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Raised in
Mouse
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Antibody s target
Synaptophysin
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Primary research fields
Neuroscience, Cell Structure, Pre-Synaptic Markers
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Brandname
None
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Antibodies applications
WB, IHC
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Antibody s reactivity
Human
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Antibody s dilutions
WB (1:1000), IHC (1:1000); optimal dilutions for assays should be determined by the user.
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Purity
Protein G Purified
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Antibody buffer for storage
PBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects ~38kDa.
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Storage recommendations
-20ºC
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Shipping recommendations
Blue Ice or 4ºC
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Antibody certificate of analysis
1 µg/ml of SMC-178 was sufficient for detection of Synaptophysin in 10 µg of human brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasmic Vesicle , Secretory Vesicle , Synaptic Vesicle Membrane , Cell Junction , Synapse
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Tissue specificity
See included datasheet or contact our support service
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Scientific context
Synaptophysin is a synaptic vesicle glycoprotein with four transmembrane domains weighing 38kDa (1). It is present in neuroendocrine cells and in virtually all neurons in the brain and spinal cord that participate in synaptic transmission. It acts as a marker for the presynaptic vesicle, and functions in synaptic vesicle exocytosis, including cholesterol-dependent vesicle biogenesis, regulation of SNARE assembly via interaction with VAMP/synaptobrevin, formation of the fusion pore initiating neurotransmitter release, and activation of vesicle endocytosis (2, 3). This gene has also been implicated in X linked mental retardation (4).
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Bibliography
1. Johnston P.A., et al. (1989) J Biol Chem. 264: 1268-1273. 2. Thiele C., et al. (2000) Nat Cell Biol. 2: 42-49. 3. Valtorta F., et al. (2004) Bioessays 26: 445-453. 4. Tarpey P.S., et al. (2009) Nat Genet. 41(5): 535-543.
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Released date
26-Apr-2010
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Tested applications
To be tested
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Tested reactivity
To be tested
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NCBI number
NP_003170.1
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Gene number
6855
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Protein number
P08247
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Antibody s datasheet
Contact our support service
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Representative figure link
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Representative figure legend
Immunohistochemistry analysis using Mouse Anti-Synaptophysin Monoclonal Antibody, Clone EP10 (SMC-178). Tissue: Brain Slice. Species: Human. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Synaptophysin Monoclonal Antibody (SMC-178) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT. Localization: Neurons and fibers but not on the white matter. Magnification: 20x. Western Blot analysis of Human Brain showing detection of ~38 kDa Synaptophysin protein using Mouse Anti-Synaptophysin Monoclonal Antibody, Clone EP11 (SMC-178). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human Brain. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Synaptophysin Monoclonal Antibody (SMC-178) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~38 kDa. Other Band(s): 25 kDa, 20 kDa. Immunohistochemistry analysis using Mouse Anti-Synaptophysin Monoclonal Antibody, Clone EP10 (SMC-178). Tissue: Cerebellum. Species: Human. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Synaptophysin Monoclonal Antibody (SMC-178) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT. Localization: Neurons and fibers. Magnification: 20x. Immunohistochemistry analysis using Mouse Anti-Synaptophysin Monoclonal Antibody, Clone EP10 (SMC-178). Tissue: Fetal brain tissue. Species: Human. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Synaptophysin Monoclonal Antibody (SMC-178) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT. Localization: Neurons. Mouse Anti-Synaptophysin Antibody [EP10] used in Immunohistochemistry (IHC) on Human Brain Slice (SMC-178) Mouse Anti-Synaptophysin Antibody [EP11] used in Western Blot (WB) on Human Brain (SMC-178) Mouse Anti-Synaptophysin Antibody [EP10] used in Immunohistochemistry (IHC) on Human Cerebellum (SMC-178) Mouse Anti-Synaptophysin Antibody [EP10] used in Immunohistochemistry (IHC) on Human Fetal brain tissue (SMC-178)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availability
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin name
Mus musculus
