- Catalog numberSMC-178D-HRP
- Product nameMouse Anti-Human Synaptophysin (native) Monoclonal IgG1, Clone EP10
- Size100 µg
- PriceAsk For Price
- LabelHRP
- CloneEP10
- ImmunogenImmunoprecipitate of human brain
- Antibody s full descriptionMouse Anti-Human Synaptophysin Monoclonal IgG1 Antibody, Clone: EP10: HRP
- Antibody s categoryMonoclonal Antibodies
- Antibody s other nameSYPH Antibody, Sypl Antibody, Syn p38 Antibody, Synaptophysin Antibody, Major synaptic vesicle protein p38 Antibody
- Raised inMouse
- IsotypeIgG1, IgG1
- Antibody s targetSynaptophysin
- Primary research fieldsNeuroscience, Cell Structure, Pre-Synaptic Markers
- BrandnameNone
- Antibodies applicationsWB, IHC
- Antibody s reactivityHuman
- Antibody s dilutionsWB (1:1000), IHC (1:1000); optimal dilutions for assays should be determined by the user.
- PurityProtein G Purified
- Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
- Antibody s concentration1 mg/ml
- Antibody s specificityDetects ~38kDa.
- Storage recommendations-20ºC
- Shipping recommendationsBlue Ice or 4ºC
- Antibody certificate of analysis1 µg/ml of SMC-178 was sufficient for detection of Synaptophysin in 10 µg of human brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
- Antibody in cellCytoplasmic Vesicle , Secretory Vesicle , Synaptic Vesicle Membrane , Cell Junction , Synapse
- Tissue specificitySee included datasheet or contact our support service
- Scientific contextSynaptophysin is a synaptic vesicle glycoprotein with four transmembrane domains weighing 38kDa (1). It is present in neuroendocrine cells and in virtually all neurons in the brain and spinal cord that participate in synaptic transmission. It acts as a marker for the presynaptic vesicle, and functions in synaptic vesicle exocytosis, including cholesterol-dependent vesicle biogenesis, regulation of SNARE assembly via interaction with VAMP/synaptobrevin, formation of the fusion pore initiating neurotransmitter release, and activation of vesicle endocytosis (2, 3). This gene has also been implicated in X linked mental retardation (4).
- Bibliography1. Johnston P.A., et al. (1989) J Biol Chem. 264: 1268-1273. 2. Thiele C., et al. (2000) Nat Cell Biol. 2: 42-49. 3. Valtorta F., et al. (2004) Bioessays 26: 445-453. 4. Tarpey P.S., et al. (2009) Nat Genet. 41(5): 535-543.
- Released date26-Apr-2010
- Tested applicationsTo be tested
- Tested reactivityTo be tested
- NCBI numberNP_003170.1
- Gene number6855
- Protein numberP08247
- Antibody s datasheetContact our support service
- Representative figure link
- Representative figure legendImmunohistochemistry analysis using Mouse Anti-Synaptophysin Monoclonal Antibody, Clone EP10 (SMC-178). Tissue: Brain Slice. Species: Human. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Synaptophysin Monoclonal Antibody (SMC-178) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT. Localization: Neurons and fibers but not on the white matter. Magnification: 20x. Western Blot analysis of Human Brain showing detection of ~38 kDa Synaptophysin protein using Mouse Anti-Synaptophysin Monoclonal Antibody, Clone EP11 (SMC-178). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human Brain. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Synaptophysin Monoclonal Antibody (SMC-178) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~38 kDa. Other Band(s): 25 kDa, 20 kDa. Immunohistochemistry analysis using Mouse Anti-Synaptophysin Monoclonal Antibody, Clone EP10 (SMC-178). Tissue: Cerebellum. Species: Human. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Synaptophysin Monoclonal Antibody (SMC-178) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT. Localization: Neurons and fibers. Magnification: 20x. Immunohistochemistry analysis using Mouse Anti-Synaptophysin Monoclonal Antibody, Clone EP10 (SMC-178). Tissue: Fetal brain tissue. Species: Human. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Synaptophysin Monoclonal Antibody (SMC-178) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT. Localization: Neurons. Mouse Anti-Synaptophysin Antibody [EP10] used in Immunohistochemistry (IHC) on Human Brain Slice (SMC-178) Mouse Anti-Synaptophysin Antibody [EP11] used in Western Blot (WB) on Human Brain (SMC-178) Mouse Anti-Synaptophysin Antibody [EP10] used in Immunohistochemistry (IHC) on Human Cerebellum (SMC-178) Mouse Anti-Synaptophysin Antibody [EP10] used in Immunohistochemistry (IHC) on Human Fetal brain tissue (SMC-178)
- Warning informationNon-hazardous
- Country of productionCanada
- Total weight kg1.4
- Net weight g0.1
- Stock availabilityIn Stock
- Other related products
- Gene targetSynaptophysin native Monoclonal IgG1 EP10
- Hostmouse
- isotype filter
- IgG1
- Short nameMouse Anti- Synaptophysin (native) Monoclonal IgG1, Clone EP10
- label filter
- HRP
- Clone nameEP10
- technique filter
- anti-Human
- anti-
- Mouse
- anti
- Techniqueanti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
- Alternative techniquemurine, antibodies