Mouse Anti-Human SOD (EC) Monoclonal IgG1 Kappa, Clone 4GG11G6
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Catalog numberSMC-167D-FITC
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Price:
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Size100 µg
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Clone4GG11G6
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ImmunogenHuman extracellular SOD purified from aortas
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Antibody s full descriptionMouse Anti-Human SOD3 Monoclonal IgG1 Kappa Antibody, Clone: 4GG11G6: FITC
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameEC SOD antibody, EC-SOD antibody, Extracellular superoxide dismutase [Cu Zn] antibody, Extracellular superoxide dismutase [Cu-Zn] antibody, Extracellular superoxide dismutase antibody, Extracellular superoxide dismutase precursor antibody, MGC20077 antibody, SOD 3 antibody, SOD3 antibody, SODE_HUMAN antibody, Superoxide dismutase 3 extracellular antibody
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Raised inMouse
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Antibody s targetSOD3
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Primary research fieldsCancer, Oxidative Stress
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BrandnameNone
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Antibodies applicationsWB, IHC, ICC/IF, ELISA
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Antibody s reactivityHuman, Mouse, Rat, Guinea Pig (Cavia porcellus)
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Antibody s dilutionsWB (1:1000), IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects extracellular SOD ~35kDa.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysis1 µg/ml of SMC-167 was sufficient for detection of EC-SOD in 20 µg of human cartilage lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellExtracellular Space
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextSuperoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2ˉ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2, 5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are three types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space (6). SOD3 can also be distinguished by its heparin-binding capacity (1).
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Bibliography1. Adachi T., et al. (1992) Clin Chim Acta. 212: 89-102. 2. Barrister J.V., et al. (1987). Crit. Rev. Biochem. 22:111-180. 3. FurukawaY., and O’Halloran T. (2006) Antioxidants & Redo Signaling. 8(5): 6. 4. Gao B., et al. (2003) Am J Physiol Lung Cell Mol Physiol 284: L917-L925. 5. Hassan H.M. (1988) Free Radical Biol. Med. 5: 377-385. 6. Wispe J.R., et al. (1989) BBA. 994: 30-36. 7. Regan, E. et al. (2005) Arthritis & Rheumatism 52(11): 3479–3491
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Released date1-May-2009
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PubMed number21112335, 20887783
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Tested applicationsWestern Blot, Immunohistochemistry
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Tested reactivityGuinea-pig, Human
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NCBI numberNP_003093.2
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Gene number6649
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Protein numberP08294
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-SOD3 Monoclonal Antibody, Clone 4GG11G6 (SMC-167). Tissue: cartilage. Species: Human. Primary Antibody: Mouse Anti-SOD3 Monoclonal Antibody (SMC-167) at 1:1000. Western Blot analysis of Human cartilage lysates showing detection of SOD3 protein using Mouse Anti-SOD3 Monoclonal Antibody, Clone 4GG11G6 (SMC-167). Primary Antibody: Mouse Anti-SOD3 Monoclonal Antibody (SMC-167) at 1:1000. Left: Control, Middle: Young cartilage, Right: Cartilage sample with osteoarthritis-arthritis.. Mouse Anti-SOD3 Antibody [4GG11G6] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human cartilage (SMC-167) Mouse Anti-SOD3 Antibody [4GG11G6] used in Western Blot (WB) on Human cartilage lysates (SMC-167)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestStressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin nameMus musculus
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Gene target
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Gene symbolSOD3
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Short nameMouse Anti- SOD (EC) Monoclonal IgG1 Kappa, Clone 4GG11G6
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Techniqueanti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG1 Kappa, IgG1
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LabelFITC
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SpeciesHuman, Humans, Mouses
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Alternative nameMouse Antibody toHuman antioxidant enzyme (EC) monoclonal IgG1 Kappa, clonality 4GG11G6
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Alternative techniquemurine, antibodies
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Clone name4GG11G6
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Gene info
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Identity
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Gene
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Long gene namesuperoxide dismutase 3
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Synonyms gene name
- superoxide dismutase 3, extracellular
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Synonyms
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Locus
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Discovery year1988-05-08
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Entrez gene record
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Classification
- Superoxide dismutases
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data