Antibody s other name
EC SOD antibody, EC-SOD antibody, Extracellular superoxide dismutase [Cu Zn] antibody, Extracellular superoxide dismutase [Cu-Zn] antibody, Extracellular superoxide dismutase antibody, Extracellular superoxide dismutase precursor antibody, MGC20077 antibody, SOD 3 antibody, SOD3 antibody, SODE_HUMAN antibody, Superoxide dismutase 3 extracellular antibody
Scientific context
Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2ˉ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2, 5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are three types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space (6). SOD3 can also be distinguished by its heparin-binding capacity (1).
Bibliography
1. Adachi T., et al. (1992) Clin Chim Acta. 212: 89-102. 2. Barrister J.V., et al. (1987). Crit. Rev. Biochem. 22:111-180. 3. FurukawaY., and O’Halloran T. (2006) Antioxidants & Redo Signaling. 8(5): 6. 4. Gao B., et al. (2003) Am J Physiol Lung Cell Mol Physiol 284: L917-L925. 5. Hassan H.M. (1988) Free Radical Biol. Med. 5: 377-385. 6. Wispe J.R., et al. (1989) BBA. 994: 30-36. 7. Regan, E. et al. (2005) Arthritis & Rheumatism 52(11): 3479–3491
Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SOD3 Monoclonal Antibody, Clone 4GG11G6 (SMC-167). Tissue: cartilage. Species: Human. Primary Antibody: Mouse Anti-SOD3 Monoclonal Antibody (SMC-167) at 1:1000. Western Blot analysis of Human cartilage lysates showing detection of SOD3 protein using Mouse Anti-SOD3 Monoclonal Antibody, Clone 4GG11G6 (SMC-167). Primary Antibody: Mouse Anti-SOD3 Monoclonal Antibody (SMC-167) at 1:1000. Left: Control, Middle: Young cartilage, Right: Cartilage sample with osteoarthritis-arthritis.. Mouse Anti-SOD3 Antibody [4GG11G6] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human cartilage (SMC-167) Mouse Anti-SOD3 Antibody [4GG11G6] used in Western Blot (WB) on Human cartilage lysates (SMC-167)
Technique
anti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
